07-2101-M

Anti-phospho PIX Antibody (Ser340)

from rabbit, purified by affinity chromatography

• 동의어(들): Rho guanine nucleotide exchange factor (GEF) 7, PAK-interacting exchange factor beta, guanine nucleotide exchange factor 7, rho guanine nucleotide exchange factor 7, SH3 domain-containing proline-rich protein, p85, PAK3, Beta-Pix, P85COOL1, COOL-1, P85,
• UNSPSC 코드: 12352203
• eCl@ss: 32160702

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: mouse
• 종 반응성(상동성에 의해 예측): rat (based on 100% sequence homology), human (based on 100% sequence homology)
• 기술: western blot: suitable
• 동형: IgG
• NCBI 수납 번호: NP_001106989
• UniProt 수납 번호: Q9ES28
• 타겟 번역 후 변형: phosphorylation (pSer340)
• 유전자 정보: human ... PAK3(5063)

설명

일반 설명

p-21-activated kinase (PAK) interacting exchange factor (PIX) is a guanine nucleotide exchange factor. PIX is a necessary component for the recruitment, regulation and activation of Rac1, as it is able to directly bind Rac1 via its SH3 domain. Studies indicate that PIX may also have an active role as a positive regulator of apoptosis, an inducer of membrane ruffling and in cell migration. Along with Rac1, PIX has been shown to regulate and interact with GIT1 and NOD2. Alternative splicing and initiation give rise to 8 known isoforms. Expression of PIX has been localized to the central nervous system. Isoforms B, C and E have highest expression in the testis and brain.

특이성

This antibody recognizes PIX phosphorylated at Ser340.

면역원

Epitope: Phosphorylated Ser340

KLH-conjugated linear peptide corresponding to mouse PIX phosphorylated at Ser340.

애플리케이션

This phospho PIX antibody is validated for use in WB for the detection of the phospho PIX protein.

품질

Evaluated by Western Blot in PIX transfected cell lysates.

Western Blot Analysis: A 1:1000 dilution of this antibody detected PIX on 10 µg of Pix transfected cell lysates.

표적 설명

~ 75 kDa

물리적 형태

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide, 1 mg/mL BSA and 30% glycerol.

분석 메모

Control
PIX transfected cell lysates

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1