07-1485

Anti-JAK1 Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): Janus Kinase 1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: rat, mouse, human
• 기술: western blot: suitable
• 동형: IgG
• NCBI 수납 번호: NM_002227.2
• UniProt 수납 번호: P23458
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... JAK1(3716)

설명

일반 설명

JAKs are non-receptor tyrosine kinases which couple to cytokine receptors, and are activated by cytokines. They regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. JAK kinases (JAK1, JAK2, JAK3, Tyk2) have a kinase domain and a second, pseudokinase domain which is not enzymatically active. The principal effectors of JAKs are the STATs (Signal Transducers and Activators of Transcription), which are direct activators of transcription, but JAKs also signal to the Ras-MAP Kinase pathway, and through Tec, to the PI3 Kinase pathway. Immunological stimuli, such as interferons and cytokines, induce recruitment of STAT transcription factors to cytokine receptor-associated JAK2. JAK2 then phosphorylates proximal STAT transcription factors, which subsequently dimerize and translocate to the nucleus where they bind DNA. The canonical JAK/Stat pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival and host resistance to pathogens. Altering JAK/Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies. Within the JAK2 kinase domain, there is a region that has considerable sequence homology to the regulatory region of the insulin receptor. Among a variety of sites, Tyrosines 1007 and 1008 (Tyr1007/1008) are sites of trans- or autophosphorylation in vivo and in vitro kinase reactions. JAK2 is required for signaling through a different subset of cytokine receptors, while JAK1 is essential for signaling from the IL-2, IL-6 and interferon receptors. JAK3 is required for signaling through cytokine receptors which share a common -chain.

면역원

Synthetic peptide corresponding to amino acids 1123-1141 of human JAK1 (CQPSNRTSFQNLIEGFEALLK)

애플리케이션

Detect JAK1 using this Anti-JAK1 Antibody validated for use in WB.

품질

Routinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected JAK1 on 10 μg of PC12 lysates.

표적 설명

130 kDa

결합

Replaces: AB3894

물리적 형태

Purified rabbit polyclonal IgG in 0.02 M Phosphate with 0.15 M NaCl, pH 7.6 and 0.1% sodium azide as a preservative.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable