06-942-AF555

Anti-acetyl-Histone H3 (Lys9) Antibody, Alexa Fluor^™ 555 Conjugate

from rabbit, ALEXA FLUOR^™ 555

• 동의어(들): H3K9ac, Histone H3, Lys9-acetylated, H3 histone family, member T, Lys9-acetylated, Lys9-acetylated histone 3, histone cluster 3, Lys9-acetylated, Lys9-acetylated H3
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 결합: ALEXA FLUOR^™ 555
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 종 반응성: rat, mouse, human
• 기술: flow cytometry: suitable; immunocytochemistry: suitable
• NCBI 수납 번호: NP_003520
• UniProt 수납 번호: P68431
• 배송 상태: wet ice
• 타겟 번역 후 변형: acetylation (Lys9)
• 유전자 정보: human ... HIST1H3F(8968)

설명

일반 설명

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.

면역원

KLH-conjugated synthetic peptide (ARTKQTARK(Ac)STG-C) corresponding to amino acids 1-12 of histone H3 with acetylated Lys9.

애플리케이션

Anti-acetyl-Histone H3 (Lys9) Antibody, Alexa Fluor^™ 555 Conjugate, Cat. No. 06-942-AF555, is a highly specific rabbit polyclonal antibody that targets histone H3 Lys9 acetylation (H3K9ac) and has been tested in Flow Cytometry and Immunocytochemistry.

Flow Cytometry Analysis: 2 µL from a representative lot detected cell population with histone H3 Lys9 acetylation (H3K9ac) among one million HEK293 cells.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected nuclear histone H3 Lys9 acetylation (H3K9ac) in A431, HeLa, and NIH/3T3 cells.

The unconjugated antibody (Cat. No. 06-492) is shown to be suitable also for Western blotting, dot blot, flow cytometry, chromatin immunoprecipitation (ChIP) and ChIP-seq applications.

품질

Evaluated by Immunocytochemistry in HUVECs.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected nuclear histone H3 Lys9 acetylation (H3K9ac) in HUVECs.

표적 설명

~17 kDa observed.

물리적 형태

Purified rabbit polyclonal antibody in PBS with 1.5% BSA with 0.05% sodium azide.

기타 정보

Concentration: Please refer to lot specific datasheet.

법적 정보

ALEXA FLUOR is a trademark of Life Technologies

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable