추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
serum
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
Tetrahymena sp., human, eukaryotes
제조업체/상표
Upstate®
기술
ChIP: suitable
immunocytochemistry: suitable
western blot: suitable
동형
IgG
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
acetylation (Lys5,Lys8,Lys 12,Lys16)
유전자 정보
human ... H4C1(8359)
일반 설명
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
특이성
Acetylated histone H4, acetylated histone H2B from Tetrahymena and weakly cross-reacts with acetylated histone H2B from HeLa cells, may cross-react with other acetylated proteins.
Other species not tested, but expected to cross-react since Histone H4 is well conserved.
면역원
Epitope: a.a. 2-19
KLH-conjugated peptide corresponding to a.a. 2-19 of Tetrahymena histone H4 [AGGAcKGGAcKGMGAcKVGAAcKRHS-C], acetylated on lysines 5, 8, 12 and 16.
애플리케이션
Chromatin Immunoprecipitation:
5-10 μL of this lot immunoprecipitated transcriptionally active chromatin containing acetylated histone H4 from 2 X 106 serum stimulated HeLa cells.
5-10 μL of this lot immunoprecipitated transcriptionally active chromatin containing acetylated histone H4 from 2 X 106 serum stimulated HeLa cells.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Use Anti-acetyl-Histone H4 Antibody (Rabbit Polyclonal Antibody) has been published and validated in ChIP, WB, ICC to detect acetyl-Histone H4 also known as H4 histone family member A, histone 1-H4a.
품질
Routinely evaluated by western blot on Tetrahymena macronuclei or acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate.
Western Blot Analysis:
1:2000 dilution of this lot detected acetylated histone H4 in acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate. Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H4.
Western Blot Analysis:
1:2000 dilution of this lot detected acetylated histone H4 in acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate. Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H4.
표적 설명
10 kDa
물리적 형태
Unpurified
Whole antiserum containing 0.05% sodium azide.
저장 및 안정성
Stable for 1 year at -20°C from date of receipt.
Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
분석 메모
Control
TSA-treated Jurkat cells. For a negative control, perform a no-antibody immunoprecipitation by incubating the supernatant fraction with 60μl of Salmon Sperm DNA/Protein A Agarose- 50% Slurry. Transcriptionally unactivated DNA samples should be prepared as controls for PCR.
TSA-treated Jurkat cells. For a negative control, perform a no-antibody immunoprecipitation by incubating the supernatant fraction with 60μl of Salmon Sperm DNA/Protein A Agarose- 50% Slurry. Transcriptionally unactivated DNA samples should be prepared as controls for PCR.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
E C Forsberg et al.
Proceedings of the National Academy of Sciences of the United States of America, 97(26), 14494-14499 (2000-12-20)
We have defined the histone acetylation pattern of the endogenous murine beta-globin domain, which contains the erythroidspecific beta-globin genes. The beta-globin locus control region (LCR) and transcriptionally active promoters were enriched in acetylated histones in fetal liver relative to fetal
Ordered recruitment of histone acetyltransferases and the TRAP/Mediator complex to thyroid hormone-responsive promoters in vivo.
Sharma, D; Fondell, JD
Proceedings of the National Academy of Sciences of the USA null
The Polycomb Ezh2 methyltransferase regulates muscle gene expression and skeletal muscle differentiation.
Caretti, G; Di Padova, M; Micales, B; Lyons, GE; Sartorelli, V
Genes & Development null
Mitotic partitioning and selective reorganization of tissue-specific transcription factors in progeny cells.
Zaidi, SK; Young, DW; Pockwinse, SM; Javed, A; Lian, JB; Stein, JL; van Wijnen, AJ; Stein, GS
Proceedings of the National Academy of Sciences of the USA null
STAT3-dependent enhanceosome assembly and disassembly: synergy with GR for full transcriptional increase of the alpha 2-macroglobulin gene.
Lerner, L; Henriksen, MA; Zhang, X; Darnell, JE
Genes & Development null
활성 필터
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
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