생물학적 소스
rabbit
Quality Level
결합
unconjugated
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
69-10C-2-10C-18, monoclonal
종 반응성
human
기술
flow cytometry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
phosphorylation (pSer70)
유전자 정보
human ... BCL2(596)
일반 설명
The Bcl name is derived from B-cell chronic lymphocytic leukemia, and was given to genes involved in that disease. Members of the Bcl-2 family share homologous domains (BH) through which they are able to homo- and hetero-dimerize. Bcl-2 plays an important role in cytochrome c release from the mitochondria; it also binds Apaf1, inhibiting Apaf-1 association with caspase-9. Bcl-2 overexpression in indicated in a number of human cancers, including melanoma, breast, prostate, and lung, and it correlates with resistance to cancer treatment (presumably due to decreased apoptosis). Bcl-2 has also been implicated in schizophrenia and autoimmune disorders. Bcl-2 phosphorylated at Ser-70 plays a critial role in the translocation of Bax from the cytosol to the mitochondria, and this may regulate NO-induced neuronal apoptosis. The molecular mechanism of Bcl-2 phosphorylation and its relationship to Bax is largely unknown.
특이성
This antibody recognizes phosphorylated Bcl-2 at Ser70.
면역원
Epitope: Ser70
KLH-conjugated linear peptide corresponding to human phosphorylated Bcl-2 at Ser70.
애플리케이션
Detect phospho-Bcl-2 (Ser70) using this Anti-phospho-Bcl-2 Antibody, clone 69-10C-2-10C-18 validated for use in WB, IP, FC.
Immunoprecipitation Analysis: 1ug from a previous lot immunoprecipitated phosphorylated Bcl-2 from 100 ug of jurkat lysate treated with paciltaxel. Arrow indicates phospho-Bcl-2 (~26kD).
Flow Cytometry Analysis: 0.1ug/ml from a previous lot was used to detect Bcl-2 in Jurkat cell lysate.
Flow Cytometry Analysis: 0.1ug/ml from a previous lot was used to detect Bcl-2 in Jurkat cell lysate.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
BCL2 & Inhibition
BCL2 & Inhibition
품질
Evaluated by Western Blot in paclitaxel treated and untreated Jurkat cell lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody detected Bcl-2 on 10 µg of Jurkat cell lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody detected Bcl-2 on 10 µg of Jurkat cell lysate.
표적 설명
26 kDa
물리적 형태
Protein A purified
Purified rabbit monoclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide and 40% Glycerol.
저장 및 안정성
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
분석 메모
Control
Paclitaxel treated and untreated Jurkat cell lysate
Paclitaxel treated and untreated Jurkat cell lysate
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Contribution of Bcl-2 phosphorylation to Bak binding and drug resistance.
Dai, Haiming, et al.
Cancer Research, 73, 6998-7008 (2013)
Michela Guglielmotto et al.
Autophagy, 10(10), 1827-1843 (2014-08-20)
The role of autophagy and its relationship with apoptosis in Alzheimer disease (AD) pathogenesis is poorly understood. Disruption of autophagy leads to buildup of incompletely digested substrates, amyloid-β (Aβ) peptide accumulation in vacuoles and cell death. Aβ, in turn, has
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