05-1336-S

Anti-phospho-Histone H3 (Ser10) Antibody, clone CMA312, Trial Size

clone CMA312, Upstate^®, from mouse

• 동의어(들): H3S10P, Histone H3 (phospho Ser10), H3 Histone, family 3A
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: CMA312, monoclonal
• 종 반응성: human, vertebrates
• 제조업체/상표: Upstate^®
• 기술: ChIP: suitable; ELISA: suitable; immunocytochemistry: suitable; multiplexing: suitable; western blot: suitable
• 동형: IgG1
• NCBI 수납 번호: NP_003484.1
• UniProt 수납 번호: P84243
• 배송 상태: wet ice
• 타겟 번역 후 변형: phosphorylation (pSer10)
• 유전자 정보: human ... H3C1(8350)

설명

일반 설명

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming the nucleosome. Histones are modified post-translationally; and these modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. Phosphorylation at Ser10, which is linked to gene activation, prevents methylation at Lys9 but facilitates acetylation of H3 and H4. Phosphorylated Ser10 is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition, Ser10 phosphorylation is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at Ser10 by Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin.

특이성

Broad species cross-reactivity is expected, based on sequence homology.

This antibody specifically recognizes Histone H3 phosphorylated at Ser10.

면역원

Epitope: Phospho Ser10

Synthetic peptide corresponding to amino acids 1-19 of human Histone H3, phosphorylated on Ser10, conjugated to KLH.

애플리케이션

Anti-phospho-Histone H3 (Ser10) Antibody, clone CMA312 is a mouse monoclonal antibody for detection of phospho-Histone H3 (Ser10) also known as H3S10P, Histone H3 (phospho S10) & has been validated in WB, ELISA, ICC, Multiplexing and ChIP.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Histones

품질

Western Blot Analysis: 1 μg/mL of this antibody detected phospho-Histone H3 (Ser10) on 1 μg of colcemid-treated HeLa acid extract.

표적 설명

~17 kDa

물리적 형태

Format: Purified

Protein G Chromatography

Purified mouse monoclonal IgG in PBS with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at 2-8°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

분석 메모

Control
HeLa Acid extract lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable