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Merck
모든 사진(2)

문서

04-1664

Sigma-Aldrich

Anti-Histidine Tag Antibody, clone RM146

clone RM146, from rabbit

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.43

생물학적 소스

rabbit

Quality Level

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

RM146, monoclonal

종 반응성(상동성에 의해 예측)

all

기술

immunoprecipitation (IP): suitable
western blot: suitable

동형

IgG

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... HRG(3273)

특이성

This antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.

면역원

Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.

애플리케이션

Immunoprecipitation Analysis (IP): 0.5-2 μg/mL from a representative lot detected Histidine Tag in HEK293T whole lysate control and IP by clone RM146.
Research Category
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
This Anti-Histidine Tag Antibody, clone RM146 is validated for use in Western Blotting, Immunoprecipitation for the detection of Histidine Tag.

품질

Evaluated by Western Blotting in HEK293T cell lysate untransfected with His-Tag fusion protein X, transfected with His-Tag fusion protein X, E. coli lysate without His-Tag Protein Y, and E. coli lysate with His-Tag Protein Y.

Western Blotting Analysis (WB): 0.2 μg/mL of this antibody detected Histidine Tag in HEK293T cell lysate transfected with His-Tag fusion protein X and E. coli lysate with His-Tag Protein Y.

표적 설명

~52 and ~85 kDa observed. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Format: Purified
Protein A purified
Rabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

does not flash

Flash Point (°C)

does not flash


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