추천 제품
형태
chloroform solution
포장
pkg of 2 × 20 mL (100600C-1g)
pkg of 1 × 4 mL (100600C-100mg)
pkg of 5 × 4 mL (100600C-500mg)
제조업체/상표
Avanti Research™ - A Croda Brand
농도
25 mg/mL (100600C-100mg)
25 mg/mL (100600C-1g)
25 mg/mL (100600C-500mg)
지질 유형
lipid extracts
배송 상태
dry ice
저장 온도
−20°C
일반 설명
This product is an extract of E. coli B (ATCC 11303) grown in Kornberg Minimal media at 37°C and taken at 3/4 log growth phase. Total E. coli lipid extract is a chloroform:methanol extract of the respective tissue. This extract is partitioned against deionized water and the chloroform phase is concentrated. Polar lipid extract is the total lipid extract precipitated with acetone and then extracted with diethyl ether.
애플리케이션
E. coli Extract Polar is suitable to prepare small unilamellar vesicles, to mimic the surface of the E. coli inner membrane.
E. coli extract polar has been for the preparation of liposomes.
E. coli Extract Polar has been used:
- in unilamellar liposomes preparation for kinetic studies
- in the reconstitution of ATP-binding cassette (ABC) transporter for electron paramagnetic resonance spectroscopy studies
- in the reconstitution of molybdate transport protein (MolBC) for continuous wave-electron paramagnetic resonance studies
생화학적/생리학적 작용
E.coli Polar lipid extracts comprise phosphatidylethanolamine as major lipid component. The minor components include the phosphatidylglycerol (PG) and cardiolipin. The leaflets of cytoplasmic membrane and inner leaflet of outer membrane are the source of E. coli lipid extract. It is used for reconstituting membrane proteins and forms bilayer by vesicle fusion.
포장
30 mL Amber Narrow Mouth Glass Bottle with Screw Cap (100600C-1g)
5 mL Clear Glass Sealed Ampule (100600C-100mg)
5 mL Clear Glass Sealed Ampule (100600C-500mg)
법적 정보
Avanti Research is a trademark of Avanti Polar Lipids, LLC
또한 이 제품과 함께 일반적으로 구입
제품 번호
설명
가격
신호어
Danger
Hazard Classifications
Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3
표적 기관
Central nervous system, Liver,Kidney
Storage Class Code
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK
WGK 3
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Small substrate transport and mechanism of a molybdate ATP binding cassette transporter in a lipid environment
Rice AJ, et al.
The Journal of biological chemistry, 289(21), 15005-15013 (2014)
Methods in Cell Biology, 128, 223-241 (2015)
The
Enzymology Primer for Recombinant DNA Technology (2017)
An inducible reconstitution system for the real-time kinetic analysis of protease activity and inhibition inside the membrane
Baker RP and Urban S
Methods in Enzymology, 584, 229-253 (2017)
Emily Peterson et al.
Biochimica et biophysica acta, 1808(2), 516-521 (2010-10-26)
Amantadine-sensitive proton uptake by liposomes is currently the preferred method of demonstrating M2 functionality after reconstitution, to validate structural determination with techniques such as solid-state NMR. With strong driving forces (two decades each of both [K(+)] gradient-induced membrane potential and
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
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