C9407

Collagenase from Clostridium histolyticum

powder, Suitable for the digestion and isolation of physiologically active pancreatic islet cells, suitable for cell culture

• 동의어(들): Clostridiopeptidase A
• CAS Number: 9001-12-1
• 효소 위원회 번호: 3.4.24.3 (BRENDA, IUBMB)
• EC Number: 232-582-9
• MDL number: MFCD00130830
• UNSPSC 코드: 12352204
• NACRES: NA.75

속성

• 생물학적 소스: Clostridium histolyticum
• Quality Level: 200
• 양식: powder
• 특이 활성도: ≥800 units/mg solid
• 분자량: 68-130 kDa
• 포장: pkg of 1 μmol
• 기술: cell culture | mammalian: suitable
• pH: 7.4
• 저장 온도: −20°C

설명

일반 설명

Collagenase enzyme from Clostridium histolyticum has collagen binding domain (CBD) and polycystic kidney disease-like (PKD-like) domains. It also has Ca²⁺ binding site for calcium binding, which is crucial for its functionality.[1] Collagenase is used in the management of connective tissue disorder, Dupuytren′s contracture.[2]

애플리케이션

Collagenase from Clostridium histolyticum has been used in the digestion:

• of pancreatic samples for islets isolation[3]
• of pulmonary artery samples for pulmonary vascular smooth muscle cells (PVSMCs) isolation[4]
• of cartilage samples for chondrocytes isolation[5]

This product is suitable for the disaggregation of human tumor, mouse kidney, human adult and fetal brain, lung and many other epithelia tissues. It has also been shown to be effective in liver and kidney perfusion studies, digestion of pancreas, isolation of nonparenchymal rat liver cells and hepatocyte preparation. Collagenase has also been used in the preparation of arterial tissue for the study of Advanced Glycosylation End Products. This enzyme has been tested for the release of heptatocytes at a concentration of approximately 1 mg/mL. Concentrations for digestion range from 0.1 to 5 mg/mL.

생화학적/생리학적 작용

Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.

The collagenase product is a mixture of enzymes secreted by C. histolyticum, with different products differentiated by the relative ratios of the 10-18 components found in the secreted enzymes. The main components are two collagenases, clostripain, and a neutral protease. The synergistic action of these enzymes degrade collagen and other intracellular materialThe action of both collagenase enzymes and the neutral protease is necessary for effective release of cells from tissue. Various types of collagen are the natural substrates for collagenase.

주의사항

As supplied, this product is stable for one year at -20°C. There is no loss in FALGPA or protease activity in 30 days at 37°C, 50°C and -20°C. Solutions of crude collagenase are stable if frozen quickly in aliquots (at 10 mg/mL) and kept frozen at -20°C. Further freeze-thaw cycles will damage the solution. The product retains 100% activity over 7 hours when held on ice.

단위 정의

One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.

제조 메모

This proudct is prepared from Type XI (C7657) Collagenase from Clostridium histolyticum. It also contains clostripain, nonspecific neutral protease and tryptic activities. Solutions are typically prepared at 1-2 mg/mL in TESCA buffer (containing 50 mM TES, 0.36 mM Calcium chloride, pH 7.4 at 37°C.

안전성 정보

• 픽토그램: GHS08,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H315,H319,H334,H335
• 예방조치 성명서: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Faceshields, Gloves