G000594
HPLC Analysis of Bronchodilators Theophylline and Other Caffeine Metabolites in Serum on Discovery® RP-AmideC16 after SPE using Discovery® DSC-18
application for SPE, application for HPLC
About This Item
technique(s)
HPLC: suitable
solid phase extraction (SPE): suitable
test parameters
sample preparation: SPE (Solid Phase Extraction)
SPE tube/cartridge: Discovery® DSC-18, 500 mg/3 mL (52603-U)
condition: 2 mL methanol, then 2 mL water
sample preparation: 1 mL porcine serum spiked with 0.1 μg/mL, 0.50 μg/mL, or 1.0 μg/mL each analyte
sample preparation: 1 mL methanol; evaporate to dryness with nitrogen stream at room temperature; reconstitute in 200 μL mobile phase containing 0.2 μg/mL or 7-methylxanthine (IS)
sample preparation: 2 mL 5% methanol in water; dry tube 10 min with nitrogen stream
column: Discovery RP-AmideC16, 15 cm × 4.6 mm, 5 μm particles, preceded by a 2 cm RP-AmideC16 guard column and 0.5 μm frit filter (505013)
mobile phase: [A] Methanol; [B] 1% acetic acid (17:38, A:B)
flow rate: 1 mL/min
column temp.: 30 °C
detector: UV, 272 nm
injection: 20 μL reconstituted porcine serum extract
suitability
application for HPLC
application for SPE
application(s)
clinical
pharmaceutical (small molecule)
Legal Information
Analyte
SPE tube or plate
analytical column
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