SRP3261
FGF-4 human
Animal-component free, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)
Synonym(s):
HBGF-4, HST-1, transforming protein KS3
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
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biological source
human
recombinant
expressed in E. coli
Assay
≥95% (HPLC)
≥95% (SDS-PAGE)
form
lyophilized
potency
≥0.5 ng/mL
mol wt
19.7 kDa
packaging
pkg of 25 μg
impurities
<0.1 EU/μg endotoxin, tested
color
white to off-white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... FGF4(2249)
General description
The FGF4 (fibroblast growth factor 4) gene is mapped to human chromosome 11q13.3. It is expressed during early embryonic development. In adults, it is strongly expressed in the testis. The encoded protein mainly interacts with FGFR1c (FGF receptor 1c) and FGFR2c. It also binds to FGFR3c and FGFR4, but to a lesser extent.
Application
- FGF-4 human has been used to study the effect of FGF4 on the differentiation of PDMCs (placenta-derived multipotent cells) into hepatocyte-like cells.
- It has been used as a growth factor to study the proliferation and differentiation of human bone marrow mesenchymal stem cells (hMSCs) to chondrocytes.
- It has been used to study hepatic differentiation.
Biochem/physiol Actions
FGF4 (fibroblast growth factor 4) was first identified as an oncogene transforming NIH3T3 (mouse embryo fibroblast cell line) cells. It helps in maintaining the self-renewal capability of normal stem cells (for instance, trophoblastic stem cells). It is also required for cellular differentiation, morphogenesis and proliferation. It controls proliferation and differentiation of normal stem cells, progenitor cells and cancer cells.
Physical form
Lyophilized from 10 mM Sodium Phosphate, pH 7.6 + 100 mM NaCl.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Isolation and characterization of epithelial progenitor cells from human fetal liver.
Liu YN, et al.
Hepatology Research : the Official Journal of the Japan Society of Hepatology, 38, 103-103 (2008)
Fibroblasts induce expression of FGF4 in ovarian cancer stem-like cells/cancer-initiating cells and upregulate their tumor initiation capacity.
Yasuda K, et al.
Laboratory Investigation; a Journal of Technical Methods and Pathology, 94, 1355-1355 (2014)
Increased FGF3 and FGF4 gene dosage is a risk factor for craniosynostosis.
Grillo L, et al.
Gene, 534, 435-435 (2014)
Chondrogenic induction of human mesenchymal stem cells using combined growth factors for cartilage tissue engineering.
Bosetti M, et al.
Journal of Tissue Engineering and Regenerative Medicine, 6, 205-205 (2012)
FGF4 and retinoic acid direct differentiation of hESCs into PDX1-expressing foregut endoderm in a time- and concentration-dependent manner.
Johannesson M, et al.
PLoS ONE, 4, e4794-e4794 (2009)
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