SRE0055
Protease Inhibitor Cocktail Powder
Reagents designed and manufactured under cGMP controls suitable for use in an IVD application.
Synonym(s):
Protease Inhibitor Cocktail powder
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
Recommended Products
biological source
bovine lung
yeast
form
lyophilized powder
storage temp.
−20°C
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Specificity
Inhibits serine, cysteine, and metallo-proteases.
Application
Optimized and tested for general use.
Protease inhibitor cocktail powder has been used in immunoblotting and immunoprecipitation.
Protease inhibitor cocktail powder has been used in immunoblotting and immunoprecipitation.
Biochem/physiol Actions
This mixture contains individual components, including AEBSF at 2 mM, Aprotinin at 0.3 μM, Bestatin at 116 μM, E-64 at 14 μM, Leupeptin at 1 μM and EDTA at 1 mM. Each component has specific inhibitory properties.
AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. EDTA is an inhibitor of metalloproteases. Leupeptin acts against both serine and cystein proteases.
Protease helps to break down proteins into soluble peptides and amino acids.
AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. EDTA is an inhibitor of metalloproteases. Leupeptin acts against both serine and cystein proteases.
Protease helps to break down proteins into soluble peptides and amino acids.
Components
1 bottle in serum bottle
Components
AEBSF
Aprotinin
Bestatin
E-64
EDTA
Leupeptin
AEBSF
Aprotinin
Bestatin
E-64
EDTA
Leupeptin
Quantity
One bottle makes 100 mL of cocktail, which are sufficient to the inhibition of proteases present in approximately 20 gram of cell extract.
Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.
Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.
Preparation Note
This product is supplied as a lyophilized powder. One bottle prepares 100 mL of a cocktail solution.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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