SAB4200435

Anti-COG7 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-CDG2E, Anti-Component of oligomeric golgi complex 7
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~86 kDa
• species reactivity: mouse, rat, human
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 5-10 μg using lysates of human HeLa cells.; indirect immunofluorescence: 1-2 μg/mL using human HeLa cells.; western blot: 2.5-5.0 μg/mL using whole extracts of mouse LA-4 and rat PC12 cells.
• UniProt accession no.: P83436
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... COG7(91949) ; mouse ... Cog7(233824) ; rat ... Cog7(293456)

Description

General description

Conserved Oligomeric Golgi complex 7 (COG7) protein belongs to the COG complex. COG complex is composed of eight distinct subunits organized in two heterotrimeric groups, COG2, 3, 4 and COG5, 6, 7 which are linked via a dimeric group formed by COG1 and COG8. COG complex is an evolutionarily conserved multi-subunit protein complex. The COG7 gene is located on the human chromosome at 16p12.2.

Specificity

Anti-COG7 recognizes human, mouse and rat COG7.

Immunogen

peptide corresponding to an internal region of human COG7, conjugated to KLH. The corresponding sequence is identical in mouse and rat COG7.

Application

Anti-COG7 antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Biochem/physiol Actions

Conserved Oligomeric Golgi complex (COG) modulates membrane trafficking and maintenance of Golgi glycosylation machinery in eukaryotic cells. COG7 protein is involved in retrograde vesicular trafficking. Mutations in the COG7 gene leads to type IIe congenital disorder of glycosylation.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable