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R4903

Sigma-Aldrich

Anti-hnRNP-L antibody, Mouse monoclonal

clone 4D11, purified from hybridoma cell culture

Synonym(s):

Anti-Heterogeneous Nuclear Ribonucleoprotein-L

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About This Item

MDL number:
MFCD03845440
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

4D11, monoclonal

form

buffered aqueous solution

mol wt

antigen ~68 kDa

species reactivity

bovine, newt, chicken, human, monkey, mouse

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.25-0.5 μg/mL using total cell extract of MDBK cells

isotype

IgG1

UniProt accession no.

P14866

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... HNRNPL(3191)
mouse ... Hnrnpl(15388)

Related Categories

General description

Monoclonal Anti-hnRNP-L (mouse IgG1 isotype) is derived from the 4D11 hybridoma produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALA/c mice immunized with hnRNP proteins and purified by affinity chromatography on ssDNA-agarose. RNA polymerase II transcripts in the nucleus are in complex with several proteins called heterogeneous nuclear ribonucleoproteins (hnRNPs). hnRNPs consist of protein groups named A to U and many of these protein groups consist of more than one isoform. Group L and M are one of the most abundant groups of proteins.

Immunogen

hnRNP proteins.

Application

Anti-hnRNP-L antibody has been used in
  • immunoblotting
  • enzyme linked immunosorbent assay (ELISA)
  • immunoprecipitation
  • immunocytochemistry
  • western blotting

Biochem/physiol Actions

Heterogeneous nuclear ribonucleoproteins-L (hnRNP-L) is involved in splicing regulations, internal translation initiation of hepatitis C virus (HCV), stability of the human vascular endothelial growth factor (VEGF) mRNA under hypoxic growth conditions, and intron independent mRNA export.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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HnRNP L binds a cis-acting RNA sequence element that enables intron-dependent gene expression.
Liu X and Mertz J E
Genes & Development, 9(14), 1766-1780 (1995)
Auto-and cross-regulation of the hnRNP L proteins by alternative splicing
RossbachO, et al.
Molecular and cellular biology, 29(6), 1442-1451 (2009)
Regulation of human vascular endothelial growth factor mRNA stability in hypoxia by heterogeneous nuclear ribonucleoprotein L
Shih S C and Claffey K P
The Journal of Biological Chemistry, 274(3), 1359-1365 (1999)
Matthew S Alexander et al.
Muscle & nerve, 63(6), 928-940 (2021-03-03)
RNA-binding proteins (RBPs) play an important role in skeletal muscle development and disease by regulating RNA splicing. In myotonic dystrophy type 1 (DM1), the RBP MBNL1 (muscleblind-like) is sequestered by toxic CUG repeats, leading to missplicing of MBNL1 targets. Mounting
Gracjan Michlewski et al.
RNA (New York, N.Y.), 16(8), 1673-1678 (2010-06-24)
RNA chromatography combined with mass spectrometry represents a widely used experimental approach to identify RNA-binding proteins that recognize specific RNA targets. An important drawback of most of these protocols is the high background due to direct or indirect nonspecific binding
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