P8124

Pyoverdines

from Pseudomonas fluorescens, >90% (HPLC)

• Synonym(s): Pyoverdine Detection, Pyoverdine Protein, Siderophore Protein
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: Pseudomonas fluorescens
• Quality Level: 200
• Assay: >90% (HPLC)
• form: powder
• solubility: H₂O: ~10 mg/mL
• shipped in: wet ice
• storage temp.: −20°C

Description

Application

Pyoverdines from Pseudomonas fluorescens has been used :

• in tryptophan fluorescence quenching to determine its binding to neutrophil-gelatinase-associated lipocalin (NGAL)
• to detect pyoverdine diffusion surrounding siderophore-conjugated monobactam (MB-1)-resistant colonies in chelexed, dialyzed Mueller-Hinton broth (CDMHB)
• for synchronous fluorescence spectra of purified pyoverdine

Biochem/physiol Actions

Pyoverdines help in promoting plant growth by chelating iron and rendering it unavailable for plant pathogens. The ferri-pyoverdine complex acts as a signaling molecule inducing the production of secreted virulence factors in Pseudomonas aeruginosa.

Pyoverdines, also called pseuobactins and pyoverdins, are fluorescent siderophores that have high affinity for iron (10³² M^-1), and are synthesized by fluorescent pseudomonads under iron-deficient growth conditions. Pyoverdines were shown to prevent iron toxicity produced by iron overload in hepatocyte cultures and effectively scavenges the hydroxyl and peroxyl radicals. Pyoverdines are effective in acquiring iron from transferrin and lactoferrin. Pyoverdines are also involved in the suppression of pythium-induced damping-off of tomato and promotion of growth in some higher plants.

Physical form

Supplied as a mixture containing mainly the succinic acid (MW=1161), 2-hydroxy glutaramide (MW=1190), and succinamide (MW=1160) forms of pyoverdines.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable