P7002

Phospho(enol)pyruvic acid trisodium salt hydrate

≥97% (enzymatic), powder

• Synonym(s): 2-(Phosphonooxy)-2-propenoic acid trisodium salt, PEP
• Linear Formula: C₃H₂O₆PNa₃ · x H₂O
• CAS Number: 5541-93-5
• Molecular Weight: 233.99 (anhydrous basis)
• MDL number: MFCD00150734
• UNSPSC Code: 12352204
• PubChem Substance ID: 24898810
• NACRES: NA.32

Properties

• product name: Phospho(enol)pyruvic acid trisodium salt hydrate, ≥97% (enzymatic)
• Assay: ≥97% (enzymatic)
• form: powder
• solubility: water: 100 mg/mL, clear to slightly hazy, colorless to very faintly yellow
• storage temp.: −20°C
• SMILES string: [Na].OC(=O)C(=C)OP(O)(O)=O
• InChI: 1S/C3H5O6P.Na.H/c1-2(3(4)5)9-10(6,7)8;;/h1H2,(H,4,5)(H2,6,7,8);;
• InChI key: KZPUEZVJSLLABH-UHFFFAOYSA-N

Description

Application

Phospho(enol)pyruvic acid trisodium salt hydrate has been used in:

• several enzyme activity assays, such as PRPP synthetase (PRPPS) assay, ribokinase (RK) assay, adenine phosphoribosyltransferase (APRT) assay
• in the reaction mixture for enzyme kinetic assay to determine platelet-derived growth factor receptor α (PDGFRA) kinetic parameters
• as a component in buffer B to react with adenosine diphosphate (ADP) to form adenosine triphosphate (ATP) for energy charge measurement

Biochem/physiol Actions

Phosphoenolpyruvic acid plays a major role in the initial step of glycolysis by acting as an inhibitor of hexokinase, phosphoglucose isomerase, phosphofructokinase and aldolase.

Phospho(enol)pyruvic acid (PEP) is involved in glycolysis and gluconeogenesis. In glycolysis, PEP is metabolized by pyruvate kinase to yield pyruvate. In plants, PEP is involved in the formation of aromatic amino acids as well as in the carbon fixation pathway.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H315,H319,H335
• Precautionary Statements: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves