MAK552
Fluo-4 No Wash Calcium Assay Kit
sufficient for fluorometric test plates
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About This Item
UNSPSC Code:
41116104
NACRES:
NA.84
Recommended Products
detection method
fluorometric
relevant disease(s)
endocrinological disorders, diabetes; genitourinary disorders; neurological disorders; cancer
storage temp.
−20°C
General description
The Fluo-4 No Wash Calcium Assay Kit provides a homogeneous fluorescence-based assay for detecting the intracellular calcium mobilization. Cells expressing a GPCR of interest that signals through calcium are preloaded with Fluo-4 AM which can cross the cell membrane. Once inside the cell, the lipophilic blocking groups of Fluo-4 AM are cleaved by non-specific cell esterase, resulting in a negatively charged Fluo-4 dye that stays inside cells, and its fluorescence is greatly enhanced upon binding to calcium. When cells are stimulated with screening compounds, the receptor signals the release of intracellular calcium, which greatly increases the fluorescence of Fluo-4.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Certificates of Analysis (COA)
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