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M7539

Sigma-Aldrich

4-(4-[6-Methoxy-2-benzoxazolyl]phenyl)-1,2,4-triazolidine-3,5-dione

≥95%

Synonym(s):

MBOTAD precursor

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About This Item

Empirical Formula (Hill Notation):
C16H12N4O4
CAS Number:
Molecular Weight:
324.29
MDL number:
UNSPSC Code:
12171500

Assay

≥95%

storage temp.

2-8°C

Application

MBOTAD is a fluorescent label for vitamin D derivatives in HPLC analysis. As a reagent it is very unstable but is easily derived from this precursor through reaction with t-butyl hypochlorite.

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K Shimada et al.
Journal of chromatographic science, 32(3), 107-111 (1994-03-01)
The retention behavior of sulfates or glucuronides of provitamin D, vitamin D, and 25-hydroxyvitamin D3, together with its fluorescent derivatives, are examined with reversed-phase high-performance liquid chromatography. Inclusion chromatography using cyclodextrin as a mobile-phase additive is also used for this

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