L1412
Lysing Enzymes from Trichoderma harzianum
lyophilized powder
Synonym(s):
Glucanex®
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About This Item
UNSPSC Code:
12352204
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form
lyophilized powder
specific activity
≥10 units/g
application(s)
diagnostic assay manufacturing
storage temp.
2-8°C
Application
Lysing Enzymes from Trichoderma harzianum is suitable retrieve DNA plugs from agarose gels. It has been used:
- for protoplast isolation
- for hydrolyzing poly(1-3)-glucose of the yeast cell wall glucan prior to yeast spheroplast transformation
- for the lysis of Aspergillus niger vegetative cells
Biochem/physiol Actions
Lysing Enzymes from Trichoderma harzianum is called Glucanex. It comprised of mixture of isoenzymes with β-1, 3 glucanase activity. Lysing Enzymes from Trichoderma harzianum hydrolyzes yeast cell wall oligosaccharides to glucan for protoplast preparation. Its stability is influenced by pH changes.
Quality
Contains β-glucanase, cellulase, protease, and chitinase activities
Unit Definition
One unit will hydrolyze casein to produce color equivalent to 1.0 micromole (181 μg) of tyrosine per minute at ph 7.5 at 37 deg C (color byfolin-ciocalteu reagent).
Legal Information
A product of Novozyme Corp.
Glucanex is a registered trademark of Novozymes Corp.
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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