H8280
N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow
matrix fast-flow highly cross-linked 4% beaded agarose
Synonym(s):
N-Hydroxysuccinimidyl-Agarose
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About This Item
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form
isopropanol suspension
extent of labeling
16-23 μmol per mL
technique(s)
protein array: suitable
matrix
fast-flow highly cross-linked 4% beaded agarose
matrix active group
N-hydroxysuccinimide active ester
matrix spacer
6 atoms (when ligands are coupled by displacement of NHS)
storage temp.
2-8°C
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General description
N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow is a cross-linked and preactivated matrix obtained by mixing Sepharose 4 Fast Flow with 6-aminohexanoic acid through a spacer arm. Esterification with N-hydroxysuccinimide activates the terminal carboxyl group. Ligand-containing primary amino groups directly interact with this active ester to form a chemically very stable amide linkage.
Application
N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow has been used as a part of a magnetic particles-based visual detection system to achieve separation upon binding of the amplified gene. It has also been used to immobilize protein during pull-down assay.
Features and Benefits
- Specifically recommended for coupling of small amino-containing proteins and peptides in process-scale applications
- High level of pre-activation, which gives a high degree of substitution of the selected ligand
- NHS coupling method enables chemically stable ligand attachment
Legal Information
Sepharose is a trademark of Cytiva
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
3 - Flammable liquids
WGK
WGK 1
Flash Point(F)
53.6 °F
Flash Point(C)
12 °C
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Woo-Hyeon Byeon et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 805(2), 361-363 (2004-05-12)
Combinatorial phage display was used to discover peptides that selectively bind to the alpha-cobratoxin (neurotoxin) component of the multi-component venom of the Thai cobra, Naja kaouthia. Peptide sequences determined in this way were synthesized chemically and were covalently attached to
Seulggie Choi et al.
Molecular immunology, 48(15-16), 2207-2213 (2011-07-12)
Porphyromonas gingivalis causes periodontal diseases and its lipopolysaccharide (LPS) is considered as a major virulence factor responsible for pathogenesis. Since initial recognition of P. gingivalis LPS (Pg.LPS) in the oral cavity might be crucial for the host response, we identified
Yoo Min Park et al.
Biosensors & bioelectronics, 141, 111415-111415 (2019-06-16)
We focused on the development of a hand-held pathogen-detection device using smartphone-embedded electronic elements combined with functionalized magnetic particles (MPs) and sepharose. To perform affinity chromatography for evaluating DNA amplicons, avidin-conjugated MPs and succinimide-linked sepharose were used with biotin-primers. To
Yoo Min Park et al.
Talanta, 195, 97-102 (2019-01-11)
The current study focuses on developing a system for visually detecting an amplified bacterial (Escherichia coli O157:H7) gene using a heavy metal particle (MP) and functionalized porous sepharose gel. To functionalize DNA-specificity to the MP, an avidin-modified MP was employed
Julia V Hartig et al.
Nucleic acids research, 39(9), 3836-3851 (2011-01-20)
In Drosophila, siRNAs are classified as endo- or exo-siRNAs based on their origin. Both are processed from double-stranded RNA precursors by Dcr-2 and then loaded into the Argonaute protein Ago2. While exo-siRNAs serve to defend the cell against viruses, endo-siRNAs
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