F5273

Monoclonal Anti-Rat Kappa & Lambda Light Chains−FITC antibody produced in mouse

clone RT-39/RL-6, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Monoclonal Anti-Rat Kappa & Lambda Light Chains
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: mouse
• conjugate: FITC conjugate
• antibody form: purified immunoglobulin
• antibody product type: secondary antibodies
• clone: RT-39/RL-6, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 25 kDa
• storage condition: protect from light
• technique(s): dot immunobinding: 1:40; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40
• isotype: IgG1/IgG2a
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

The product is a mixture derived from hybridomas RT-39 and RL-6, secreting monoclonal antibodies against rat κ and λ light chains, respectively.

The product recognizes epitopes located on the rat κ and λ light chains on the various rat immunoglobulin classes and subclasses. The epitope recognized on the κ light chains is shared by both the 1a and 1b allotypes of this molecule. The antibody detects the κ and λ light chains derived from normal serum immunoglobulins or from myeloma proteins. Weak cross-reaction is observed with guinea pig immunoglobulins but not with human, bovine, cat, chicken, dog, goat, horse, mouse, pig, rabbit or sheep immunoglobulins.

Application

Monoclonal Anti-Rat Kappa & Lambda Light Chains-FITC antibody produced in mouse has been used in:

• fluorescent dot binding immunoassay (FDIBA)
• immunocytochemistry
• immunohistochemistry
• flow cytometry

Biochem/physiol Actions

FITC Monoclonal Anti-Rat kappa and lambda Light Chains may serve as an essential tool in the localization of light chains on rat immunoglobulins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 8.0, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves