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Chloroplast Isolation Kit

isolation of intact chloroplasts from leaves

Synonym(s):

Chloroplast Purification Kit, Isolation Kit for Chloroplast, Kit for Chloroplast Isolation

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

Quality Level

200

technique(s)

centrifugation: suitable

shipped in

wet ice

storage temp.

2-8°C

General description

The Chloroplast Isolation Kit provides an efficient procedure for isolating intact chloroplasts from plant leaves. The procedure involves mechanical cell wall and cell membrane breakage, removal of cell debris and leaf tissue by filtration, collection of total cell chloroplast fraction by centrifugation, and separation of intact chloroplasts from broken chloroplasts using a Percoll® layer or gradient.

Application

Chloroplast Isolation Kit may be used in chloroplast organelle research to isolate intact chloroplasts from plant leaves for structural and functional studies.

Features and Benefits

  • Specifically formulated buffer - Saves time using easy to follow simple and proven protocols
  • Isolates intact chloroplasts - Useful for studies of such processes as carbon assimilation, electron flow and phosphorylation, metabolic transport or protein targeting
  • Total organelle protein complement - Useful for functional studies, metabolic transport, proteomic profiling, and genomic applications
  • Convenient format - Saves time and reduces waste

Suitability

The Chloroplast Isolation Kit has been tested for use with spinach, pea, lettuce, cabbage, mangold, and tobacco.

Kit Components Also Available Separately

Product No.
Description
SDS
  • Chloroplast Isolation Buffer 5x (CIB) 500 mL
  • A8022Bovine Serum Albumin, heat shock fraction, pH 5.2, ≥96% 3 gSDS
  • Chloroplast Isolation Buffer 5x (CIB) 4 ea
  • P4937Percoll®, pH 8.5-9.5 (25 °C), suitable for cell culture 100 mLSDS

Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Xiaoli Luo et al.
PloS one, 8(1), e54002-e54002 (2013-01-22)
In plants, CuZn superoxide dismutase (CuZnSOD, EC l.15.1.1), ascorbate peroxidase (APX, EC 1.11.1.11), and catalase (CAT, EC l.11.1.6) are important scavengers of reactive oxygen species (ROS) to protect the cell from damage. In the present study, we isolated three homologous
The Complete Chloroplast Genome of 17 Individuals of Pest Species <I>Jacobaea vulgaris</I>: SNPs, Microsatellites and Barcoding Markers for Population and Phylogenetic Studies.
Doorduin, L., et al.
DNA Res., 1-13 (2011)
Diana V Dugas et al.
Scientific reports, 5, 16958-16958 (2015-11-26)
The Leguminosae has emerged as a model for studying angiosperm plastome evolution because of its striking diversity of structural rearrangements and sequence variation. However, most of what is known about legume plastomes comes from few genera representing a subset of
Bruna Marques Dos Santos et al.
Journal of proteomics, 150, 252-257 (2016-10-25)
Photosynthetic organisms may be drastically affected by the future climate projections of a considerable increase in CO2 concentrations. Growth under a high concentration of CO2 could stimulate carbon assimilation-especially in C3-type plants. We used a proteomics approach to test the
Paul E Abraham et al.
Nature plants, 2, 16178-16178 (2016-11-22)
Already a proven mechanism for drought resilience, crassulacean acid metabolism (CAM) is a specialized type of photosynthesis that maximizes water-use efficiency by means of an inverse (compared to C3 and C4 photosynthesis) day/night pattern of stomatal closure/opening to shift CO2
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