BYD, A human cell line

940822106, IHW Number: 9202

• UNSPSC Code: 41106514

Properties

• biological source: human blood
• ethnicity: Caucasian
• gender: female
• growth mode: Suspension
• karyotype: Not specified
• morphology: Lymphoblastoid
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice

Description

General description

Consanguineous: No

Homozygous: No

Cell Line Description

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

DNA Profile

A: 03 02
B: 35 50
HLA DRB1: 0301 1104
HLA DRB3: 0101 0202
HLA DQAP: 4.1
HLA DQA1: 501
HLA DQB1: 0201 0301
HLA DPB1: 02012 0301

Serological Profile

HLA A: 3 2
HLA A ief: 3.2 2.2
HLA B: 35 21
HLA B ief:

Culture Medium

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Subculture Routine

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Other Notes

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