Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule. For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.
COS-1 Cell Line
88031701, African green monkey kidney, Fibroblast
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
Recommended Products
Product Name
COS-1 Cell Line, 88031701, from African green monkey kidney, SV40 transformed, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
biological source
African green monkey kidney
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Monkey African green kidney, SV40 transformed
Cell Line Description
Derived from CV-1 simian cells transformed by origin-defective mutant of SV40 which codes for wildtype T Ag. Possible host for propagation of pure populations of recombinant SV40 virus. The cells should be handled under laboratory containment level 2.
Application
COS-1 cell line has been used to test the toxicity of various concentrations of iron oxide nanoparticles (for use in medical diagnostics).[1] It has also been used to study the interaction of nanoparticles with their cellular targets.[2]
Virus replication studies: SV40
Culture Medium
Subculture Routine
Split semi-confluent cultures 1:3 to 1:6, i.e., seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C. In order to prevent syncitia formation, do not allow cells become confluent.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Sorry, we don't have COAs for this product available online at this time.
If you need assistance, please contact Customer Support.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
-
How many Cell Line cells are in the vial?
1 answer-
Helpful?
-
-
Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?
1 answer-
If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.
Helpful?
-
-
What passage are my Cell Line cells?
1 answer-
If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).
Helpful?
-
-
Are my Cell Line cells mycoplasma tested?
1 answer-
ECACC routinely tests all manufactured cell banks for mycoplasma.
Helpful?
-
-
Are the Cell Line cells tested for viral pathogens?
1 answer-
HPA Cultures does not perform any viral testing on the cell lines.
Helpful?
-
-
How do I handle Cell Line frozen cells upon arrival?
1 answer-
Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.
Helpful?
-
-
What medium should I use when I thaw the Cell Line cells?
1 answer-
The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
