AFRC MAC 77

85102262, Reactivity: Anti-Nitrate Reductase

• UNSPSC Code: 41106514

Properties

• biological source: Rat x Rat Hybridoma
• ImmunoDon: F344 rat spleen
• Myeloma: Y3Ag1.2.3
• Reactivity: Anti-Nitrate Reductase
• technique(s): cell culture | mammalian: suitable
• isotype: IgG2b
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Rat Y3Ag1.2.3 x Rat F344 spleen

Cell Line Description

The antibody specific for NADH nitrate reductase and inhibits the reduction of nitrate to nitrite using NADH as electron donor. It also inhibits the reduction of nitrate using flavin nucleotides (FMNH2 or FADH2) and methyl viologen. The antibody is directed against NADH-NR of mono and dicotyledons.

Immunogen

Nitrate reductase purified from the leaves of 6 to 8 week old spinach plants - Spinacea oleracea l

Culture Medium

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Other Notes

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