An undifferentiated carcinoma cell line established from a forehead metastasis of a malignant teratoma. This is not an embryonal carcinoma cell line. This cell line is tumourigenic in nude mice.
Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin or trypsin/EDTA; 10% CO2; 37°C.
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