C1872

Anti-CPSF2 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-100-KD subunit, Anti-CPSF100, Anti-Cleavage and polyadenylation specificity factor 2
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• form: buffered aqueous solution
• mol wt: antigen ~100 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 2-5 μg using A20 cell lysates; western blot: 2-4 μg/mL using K562 cell lysates
• UniProt accession no.: Q9P2I0
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... CPSF2(53981) ; mouse ... Cpsf2(51786)

Description

Immunogen

synthetic peptide corresponding to amino acids 718-732 of human CPSF2 conjugated to KLH. The corresponding sequence is identical in mouse.

Application

Anti-CPSF2 (C-terminal) antibody produced in rabbit is suitable for immunoprecipitation at a concentration of 2-5μg using A20 cell lysates and western blotting at a concentration of 2-4μg/mL using K562 cell lysates.

Biochem/physiol Actions

Cleavage and polyadenylation specificity factor subunit 2 is a protein encoded by the CPSF2 gene in humans. It is also referred to as CPSF100. CPSF and cleavage stimulation factor (CstF) play an important role during the formation of the 3′ ends of mRNA for 3′ cleavage of the transcript as well as for subsequent polyadenylation. CPSF and poly(A) polymerase (PAP) activity is controlled by Eg2-catalyzed phosphorylation during formation of cytoplasmic polyadenylation complex.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves