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A5854

Sigma-Aldrich

Anti-AFX (FOXO4) antibody, Mouse monoclonal

clone AF3.10, purified from hybridoma cell culture

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About This Item

MDL number:
MFCD03791410
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AF3.10, monoclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

human

technique(s)

immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using COS-7 cell extracts expressing human AFX

isotype

IgG2b

UniProt accession no.

P98177

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FOXO4(4303)

General description

Forkhead box O4 (FOXO4) belongs to the FOXO transcription factor family that regulates growth, cell cycle, metabolism, stress tolerance, aging and apoptosis in cells. Studies have reported that USP7 modulates FOXO4 transcription. Moreover, it has been demonstrated that peroxide stress acetylates FOXO4, whereas the longevity protein hSir2SIRT1 deacetylates it.

Specificity

Monoclonal Anti-AFX (FOXO4) recognizes human AFX.

Application

Monoclonal Anti-AFX (FOXO4) antibody can be used for western blot assays at 0.5-1μg/ml. The antibody is also suitable for use in indirect ELISA, immunocytochemistry and microarray applications.

Biochem/physiol Actions

Anti-AFX (FOXO4) antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the hybridoma AF3.10 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice. AFX plays a central role in the regulation of cell proliferation. It integrates signals from PI3K/PKB and Ras/Ral signaling pathways to regulate transcription of the cell cycle inhibitor p27kip1. AFX activation results in increased expression of p27kip1, resulting in cell cycle arrest. Overexpression of AFX blocks cells cycle progression at G1 by a p27kip1 dependent mechanism.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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AFX-like Forkhead transcription factors mediate cell-cycle regulation by Ras and PKB through p27 kip1
Medema RH, et al.
Nature, 404(6779), 782-782 (2000)
Armando van der Horst et al.
The Journal of biological chemistry, 279(28), 28873-28879 (2004-05-06)
FOXO transcription factors have important roles in metabolism, cellular proliferation, stress tolerance, and aging. FOXOs are negatively regulated by protein kinase B/c-Akt-mediated phosphorylation. Here we show that FOXO factors are also subject to regulation by reversible acetylation. We provide evidence
Armando van der Horst et al.
Nature cell biology, 8(10), 1064-1073 (2006-09-12)
FOXO (Forkhead box O) transcription factors are important regulators of cellular metabolism, cell-cycle progression and cell death. FOXO activity is regulated by multiple post-translational modifications, including phosphorylation, acetylation and polyubiquitination. Here, we show that FOXO becomes monoubiquitinated in response to

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