A5763

Adenosine 3′,5′-diphosphate disodium salt

≥96%

• Synonym(s): 3′-phosphoadenosine 5′-phosphate, 3′-phosphorylated nucleotide, PAP, 3′-Phosphoadenosine 5′-phosphate
• Empirical Formula (Hill Notation): C₁₀H₁₃N₅Na₂O₁₀P₂
• CAS Number: 75431-54-8
• Molecular Weight: 471.16
• MDL number: MFCD00057007
• UNSPSC Code: 41106305
• eCl@ss: 32160414
• PubChem Substance ID: 24891020
• NACRES: NA.51

Properties

• biological source: synthetic (inorganic)
• Quality Level: 200
• Assay: ≥96%
• form: powder
• solubility: water: 25 mg/mL, clear, colorless to very faintly yellow
• storage temp.: −20°C
• SMILES string: [Na].Nc1ncnc2n(cnc12)C3OC(COP(O)(O)=O)C(OP(O)(O)=O)C3O
• InChI: 1S/C10H15N5O10P2.Na.H/c11-8-5-9(13-2-12-8)15(3-14-5)10-6(16)7(25-27(20,21)22)4(24-10)1-23-26(17,18)19;;/h2-4,6-7,10,16H,1H2,(H2,11,12,13)(H2,17,18,19)(H2,20,21,22);;
• InChI key: ISROZYFZEAVMSP-UHFFFAOYSA-N

Description

General description

3′-phosphoadenosine 5′-phosphate (PAP), a 3′-phosphorylated nucleotide is found in almost all organisms and is obtained as a by-product of sulfur and lipid metabolism.[1]

Application

Adenosine 3′,5′-diphosphate disodium salt has been used:

• to spot sample on cellulose high-performance thin-layer chromatography (HPTLC) plates in two-dimensional thin layer chromatography[2]
• in enzyme activity assay to study the activities of HOS2/FIERY1 wild type
• hos2 mutant and fiery1?2 mutant protein against 3′-phosphoadenosine 5′-phosphate (PAP)[3]
• as a standard for the quantification of phosphoadenosines[4]

Biochem/physiol Actions

3′-phosphoadenosine 5′-phosphate (PAP) is capable of blocking exoribonucleases (XRNs) activity in the nucleus and cytosol. It stimulates stomatal closure and can serve as a secondary messenger during abscisic acid (ABA) signaling.[5] It is capable of blocking RNA catabolism. Hence it may serve as a physiological modulator of poly (ADP-ribose) polymerase 1 (PARP1) activity.[1]

Adenosine 3′,5′-diphosphate (PAP) is used to study the kinetics and mechanisms of hydroxysteroid sulfotransferases such as SULT1A1, SULT2A1 of which it is product inhibitor.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H315,H319,H335
• Precautionary Statements: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves