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94581

Sigma-Aldrich

Hirudin recombinant from yeast

lyophilized powder, white, >1500 U/vial

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About This Item

CAS Number:
8001-27-2
EC Number:
232-279-1
MDL number:
MFCD00803952
UNSPSC Code:
12352202
NACRES:
NA.77

biological source

synthetic
yeast (Pichia pastoris)

form

lyophilized powder

specific activity

>1500 U/vial

mol wt

Mr ~6963.5

color

white

solubility

0.1 M Tris, 0.2 M NaCl, pH 8.4: 1 vial/mL, clear, colorless

storage temp.

−20°C

Application

Recombinant hirudin from yeast has been used in mannan-binding lectin serine protease 2 (MASP2) activation assay. It has also been used in pre-treating the nanosheets in phosphate buffered saline (PBS) for platelet adhesion assay.

Biochem/physiol Actions

Recombinant hirudin appears to be antigenic. Independent studies have found that 50-70% of patients treated for several days with recombinant hirudin develop at least one hirudin antibody isotype. The antibodies reduce the anticoagulant effect of hirudin, but prolong its plasma half-life by reducing renal filtration.
The anticoagulant, hirudin, is the most potent natural inhibitor of both soluble and clot-bound thrombin. It binds thrombin with high affinity, covering more than 20% of its surface area and occluding both the active site and exosite I (fibrinogen and PAR recognition site). Hirudin blocks thrombus growth and platelet activation. It is not metabolized in the bloodstream of humans and is eliminated unchanged via kidney filtration.

Unit Definition

1 U corresponds to 1 ATU (antithrombin unit; 1 ATU is defined as the amount of inhibitor that neutralizes 1 NIH unit of thrombin).

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Volker Liebe et al.
Seminars in thrombosis and hemostasis, 28(5), 483-490 (2002-11-07)
This article provides an overview of the clinically relevant characteristics of antibodies directed toward recombinant (r) hirudin, with emphasis on the different ways in which these antibodies may influence pharmacokinetics and pharmacodynamics of r-hirudin. A high incidence of anti-hirudin antibody
Multiple routes of complement activation by Mycobacterium bovis BCG
Carroll MV, et al.
Molecular Immunology, 46(16), 3367-3378 (2009)
A few immobilized thrombins are sufficient for platelet spreading
Okamura Y, et al.
Biophysical Journal, 100(8), 1855-1863 (2011)

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