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A3174

Sigma-Aldrich

Bovine Serum Albumin solution

30% in saline, high avidity, contains azide and caprylate, aseptically filled

Synonym(s):

Albumin solution from bovine serum, BSA solution

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.27

biological source

bovine

Quality Level

sterility

aseptically filled

Assay

28-32% (biuret)

form

solution

quality

high avidity, contains azide and caprylate

purified by

heat shock fractionation

contains

0.1% sodium azide as preservative
10-15 mg/gram protein octanoic acid as stabilizer

origin

USA origin

concentration

28-32% (biuret)
30% in saline

technique(s)

blocking: suitable

UniProt accession no.

foreign activity

BT Virus, none detected
VSV Virus, none detected

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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General description

Bovine serum albumin (BSA) belongs to the serum albumin family. It comprises three domains with two sub-domains under each. It is an α-helical, globular and non-glycosylated protein. BSA has 17-disulfide bonds.

Application

Bovine Serum Albumin solution has been used in the blocking of nonspecific binding in brain sections prior to light microscopy studies.

Biochem/physiol Actions

Bovine Serum Albumin (BSA) is a transporter for drugs, hormones and fatty acids. It is a crucial component of the cell culture media and favors embryonic stem cells (hESC) differentiation. BSA displays high solubility and is exploited in the cosmetic and pharmaceutical industry.It is used as a blocking agent in enzyme-linked immunosorbent assay (ELISA).

Analysis Note

Tested for avidity with known incomplete antibody (not saline agglutinable).

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Cystic fibrosis transmembrane conductance regulator protein expression in the male excretory duct system during development
Marcorelles P, et al.
Human Pathology, 43(3), 390-397 (2012)
Binding of fatty acid amide amphiphiles to bovine serum albumin: role of amide hydrogen bonding
Ghosh S and Dey J
The Journal of Physical Chemistry B, 119(25), 7804-7815 (2015)
Enzyme-linked immunosorbent assay (ELISA) and blocking with bovine serum albumin (BSA)?not all BSAs are alike
Xiao Y and Isaacs SN
Journal of Immunological Methods, 384(1-2), 148-151 (2012)
Albumin and mammalian cell culture: implications for biotechnology applications
Francis GL
Cytotechnology, 62(1), 1-16 (2010)
Structural changes during the unfolding of Bovine serum albumin in the presence of urea: A small-angle neutron scattering study
Das A, et al.
Pramana, 63(2), 363-368 (2004)

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