50484
Methylene Blue solution
suitable for microbiology
Synonym(s):
Ehrlich’s reagent III, Loeffler’s Methylene Blue
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About This Item
Empirical Formula (Hill Notation):
C16H18ClN3S
CAS Number:
Molecular Weight:
319.85
Beilstein:
3641570
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
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shelf life
limited shelf life, expiry date on the label
concentration
0.5% in H2O (0.5 g/100 mL)
technique(s)
microbe id | staining: suitable
application(s)
microbiology
suitability
Bacillus spp.
Corynebacterium spp.
bacteria
SMILES string
[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
InChI
1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1
InChI key
CXKWCBBOMKCUKX-UHFFFAOYSA-M
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General description
Methylene Blue was earlier recognized as a good nuclear stain, the staining solutions are always either aged or alkalized, if not both. More importantly it is an excellent stain for ribonucleic acid, which is the important stainable constituent of bacterial bodies, of lymphocyte and hemoportoal cytoplasm, and of nerve cell tigroid granules. It can also be employed in capsule stain. The capsule stain begins as a negative stain leaving the organisms colorless. The capsule are clearly seen as halo surrounding the bacterial cell. Another staining procedure is performed with nigrosin (Cat. No. 43925).
Components
reagent contains:
methylene blue 0.5 g
distilled water 100 ml
methylene blue 0.5 g
distilled water 100 ml
replaced by
Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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