39727

Perfringens Agar Base

suitable for microbiology, NutriSelect^® Plus

• UNSPSC Code: 41171609
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 200
• form: powder
• composition: Agar, 15 g/L ; Beef extract, 5 g/L ; Ferric ammonium citrate, 1 g/L ; Papaic digest of soybean meal, 5 g/L ; Sodium metabisulphite, 1 g/L ; Tryptose, 15 g/L ; Yeast extract, 5 g/L
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• application(s): bioburden testing; cleaning products; cosmetics; environmental; food and beverages; personal care; microbiology
• suitability: selective and differential for Clostridium spp.

Description

General description

Perfringens Agar Base with the addition of selective supplement and enrichment is used for the presumptive identification and enumeration of Clostridium perfringens from food.

Application

Perfringens Agar Base has been used in the cell culture of Clostridium perfringens.[1]

Preparation Note

Suspend 23.5 g of Perfringens Agar Base in 475 ml of distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C. Add 25 ml of Egg Yolk Emulsion (Cat. No. 17148) and additionally the rehydrated contents of 1 vial of Perfringens S.F.P. Supplement (Cat. No. 53436) or 1 vial of Perfringens T.S.C. Supplement (Cat. No. P9352). Mix well and pour into sterile petri plates.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H319
• Precautionary Statements: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves