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21886

Sigma-Aldrich

5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate N-succinimidyl ester

for fluorescence, ≥85% (HPLC)

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About This Item

Empirical Formula (Hill Notation):
C29H17Cl2NO11
CAS Number:
147265-60-9
Molecular Weight:
626.35
MDL number:
MFCD00037456
UNSPSC Code:
12352200

grade

for fluorescence

Assay

≥85% (HPLC)

mp

≥220 °C (lit.)

solubility

DMF: soluble
DMSO: soluble
methanol: soluble

fluorescence

λex 470 nm; λem 529 nm in 0.1 M Tris pH 8.0 (esterase)

storage temp.

−20°C

SMILES string

CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C4(OC(=O)c5cc(ccc45)C(=O)ON6C(=O)CCC6=O)c2cc1Cl.CC(=O)Oc7cc8Oc9cc(OC(C)=O)c(Cl)cc9C%10(OC(=O)c%11ccc(cc%10%11)C(=O)ON%12C(=O)CCC%12=O)c8cc7Cl

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Amine reactive label for long term tracing of cells. Forms green fluorescent conjugates upon deacetylation

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Certificates of Analysis (COA)

Lot/Batch Number
400446/1

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C M Griffith et al.
Development (Cambridge, England), 116(4), 1087-1099 (1992-12-01)
During the fusion of rodent embryo palatal shelves, the cells of the outer epithelial layer slough off, allowing the cells of the medial edge basal layer to form a midline seam that undergoes epithelial-mesenchymal transformation, as judged by electron microscopy

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