Both plates Y04C and Y04T are suitable for long-term culture (3 days or more). However, with Y04C, yeast cells will stop dividing after approximately 24 hours, while with Y04T, cells will continue to divide. In the case of Y04T, it is primarily the "Mother" cells that are retained and continue to divide, making the plate suitable for customers interested in "split lifetime measurement." It is recommended for the end user to start with Y04C to get a feel for CellASIC and then try Y04T as needed, as Y04C would be easier to get started and use. Regarding shelf life, it is recommended to use the plate within 6 months of receipt.
Y04C-02-5PK
CellASIC ONIX plate for haploid yeast cells (4 chamber, 3.5-5 micron)
The Y04 plates for Haploid Yeast Cells utilize a microfabricated silicone ceiling with a height similar to yeast cells to restrict their growth in a single focal plane & maintaining x,y position over time.
Synonym(s):
Haploid Yeast Cell Plate, Haploid Yeast Plate, ONIX Yeast Plate
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About This Item
UNSPSC Code:
41104923
eCl@ss:
32011202
NACRES:
NB.22
Pricing and availability is not currently available. Contact Technical Service
Recommended Products
material
PMMA
glass
polycarbonate
polydimethylsiloxane (PDMS)
manufacturer/tradename
CellASIC® ONIX
W × L × H
85.48 mm × 127.76 mm × 14.35 mm
compatibility
for use with cells sized 3.5–5.0 μm
shipped in
ambient
General description
The Y04 plates for Haploid Yeast Cells utilize a microfabricated silicone ceiling with a height similar to yeast cells to restrict their growth in a single focal plane & maintaining the x,y position over time.
Application
CellASIC® ONIX plate for haploid yeast cells (4chamber, 3.5-5 micron) has been used in live-cell microscopy and analysis of Schizosaccharomyces pombe cells.[1] It has also been used in live cell imagining of Saccharomyces cerevisiae strains.[2] This CellASIC ONIX plate for haploid yeast cells (4chamber, 3.5-5 micron) is also suitable for:
- Time-lapse analysis of yeast cells
- Long-term continuous perfusion experiments
- Solution exchange experiments (induction, inhibition, drug dosing, etc.)
- Comparison of up to 4 different cell types or exposure conditions(media components) in parallel
- Cell division tracking (follow mother/daughter cells over generations)
- Temperature and gas atmospheric control (temperature shift, anoxic conditions etc.)
Features and Benefits
- Compatible with CellASIC® ONIX2 Microfluidic system and ONIX2 Manifolds
- Enables perfusion-based, long-term, live-cell analysis with solutionswitching
- The plate offers a controlled and dynamicmicroenvironment for cells
Legal Information
CELLASIC is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Spinning-disk confocal microscopy of yeast.
Kurt Thorn
Test, Volume 470, 581-602 (2010)
Distinct interactions select and maintain a specific cell fate.
Doncic A, Falleur-Fettig M, Skotheim J
Molecular Cell, Vol. 43, Issue 4, 528-539 (2011)
Dynamic analysis of cytosolic glucose and ATP levels in yeast using optical sensors.
Bermejo C, Haerizadeh F, Takanaga H, Chermak D, Frommer WB
The Biochemical Journal, 432(2), 399-406 (2010)
Oscillations in CDC14 release and sequestration reveal a circuit underlying mitotic exit.
Romilde Manzoni, Francesca Montani, Clara Visintin, Fabrice Caudron, Andres Ciliberto et al.
The Journal of cell biology, vol. 190 no. 2, 209-222 (2010)
Chia-Wei Lee et al.
Nature cell biology, 22(2), 159-166 (2020-02-08)
Nuclear pore complexes (NPCs) are very large proteinaceous assemblies that consist of more than 500 individual proteins1,2. NPCs are essential for nucleocytoplasmic transport of different cellular components, and disruption of the integrity of NPCs has been linked to aging, cancer
Related Content
Convert your microscope to a live cell imaging system – the CellASIC® ONIX2 system allows precise manipulation of the cell culture environment for dynamic live cell imaging and analysis using mammalian, yeast, and bacterial cells.
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Can Y04C plates be used for long-term experiments with yeast cells, and can an estimated average shelf life for the plates be provided if there is an anticipated plate usage for the year?
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