MABS2257

Anti-sACDY10 Antibody, clone R21

• Synonym(s): Anti-sACDY10, Clone R21 Anti-sACDY10, sACDY10 Detection Antibody
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: R21, monoclonal
• mol wt: calculated mol wt 187.15 kDa; observed mol wt ~75 kDa
• purified by: using protein G
• species reactivity: canine, rat, human, monkey, mouse
• packaging: antibody small pack of 100 μg
• technique(s): immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG1κ
• epitope sequence: N-terminal
• Protein ID accession no.: NP_060887
• UniProt accession no.: Q96PN6
• shipped in: 2-8°C
• target post-translational modification: unmodified

Description

General description

Adenylate cyclase type 10 (UniProt: Q96PN6; also known as EC:4.6.1.1, AH-related protein, Adenylate cyclase homolog, Germ cell soluble adenylyl cyclase, hsAC, sAC, Testicular soluble adenylyl cyclase) is encoded by the ADCY10 (also known as SAC) gene (Gene ID: 55811) in human. sAC catalyzes the formation of the signaling molecule cAMP. It is present in cytoplasm, nucleus, and mitochondria and is mainly detected in airway epithelial cells and testis. It is also expressed in several other tissues, but at much lower levels. It serves as a sensor to mediate responses to changes in cellular pH and bicarbonate levels and regulates pH-dependent V-ATPase recycling. sAC is shown to be activated by manganese or magnesium ions. In the presence of magnesium ions, it is activated by bicarbonate, however, in the presence of manganese ions its activity is inhibited by bicarbonate. In the absence of magnesium and bicarbonate, its activity is shown to be weakly activated by calcium ions. sAC plays a critical role in mammalian spermatogenesis by producing the cAMP, which regulates cAMP-responsive nuclear factors indispensable for sperm maturation in the epididymis. It induces capacitation, the maturational process that sperm undergo prior to fertilization and is also involved in ciliary beat regulation. sAC is shown to play a role in the pathogenesis of certain hyperproliferative skin disorders via modulation of gene expression. Its levels are shown to be upregulated in the nuclei of keratinocytes in certain hyperproliferative skin diseases, such as psoriasis and squamous cell carcinoma. However, it is shown that sAC is lost from the nucleus when a malignant epithelial tumor acquires invasive properties in the dermis. (Ref.: Zippin, JH., et al. (2010). J. Invest. Dermatol. 130(5); 1279-1287; Hess, KC., et al. (2005). Dev Cell. 9(2); 249-259; Pastor-Soler, N., et al. (2003). J. Biol. Chem. 278(49); 49523-49529).

Specificity

Clone R21 is a mouse monoclonal antibody that detects soluble adenylyl cyclase in multiple mammalian species.

Immunogen

GST-tagged fusion protein corresponding to the 50 kDa splice variant of human soluble Adenylyl cyclase (sAC).

Application

Quality Control Testing

Evaluated by Western Blotting with GST-soluble adenylate cyclase fusion protein.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected GST-soluble Adenylate cyclase type 10 fusion protein.

Tested Applicaitons

Immunohistochemistry Applications: A representative lot detected sACDY10 in Immunohistochemistry applications (Pastor-Soler, N., et al. (2003). J Biol Chem.;278(49):49523-9).

Western Blotting Analysis: A representative lot detected sACDY10 in Western Blotting applications (Zippin, J.H., et al. (2003). FASEB J.;17(1):82-4; Pastor-Soler, N., et al. (2003). J Biol Chem.;278(49):49523-9; Hess, K.C., et al. (2005). Dev Cell.;9(2):249-59; Zippin, J.H., et al. (2010). J Invest Dermatol.;130(5):1279-87).

Immunocytochemistry Analysis: A representative lot detected sACDY10 in Immunocytochemistry applications (Zippin, J.H., et al. (2003). FASEB J.;17(1):82-4; Pastor-Soler, N., et al. (2003). J Biol Chem.;278(49):49523-9; Hess, K.C., et al. (2005). Dev Cell.;9(2):249-59; Zippin, J.H., et al. (2010). J Invest Dermatol.;130(5):1279-87).

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected sACDY10 in COS-7 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected sACDY10 in human melanoma tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-sACDY10, clone R21, Cat. No. MABS2257, is a mouse monoclonal antibody that detects Adenylate cyclase type 10 (sAC) and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable