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MABS1942

Sigma-Aldrich

Anti-Perilipin-1 Antibody, clone Peri 112.17.1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

antibody form

purified antibody

clone

Peri 112.17.1
monoclonal

purified by

using protein G

species reactivity

human, bovine, mouse

concentration

(Please refer to lot specific datasheet.)

technique(s)

electron microscopy: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

UniProt accession no.

General description

KLH-conjugated linear peptide corresponding to first 20 amino acids from the N-terminal region of human Perilipin-1.

Specificity

Clone Peri 112.17.1 is a mouse monoclonal antibody that detects Perilipin-1. It targets an epitope within 20 amino acids from the N-terminal region.

Application

Immunoprecipitation Analysis: A representative lot immunoprecipitated Perilipin-1 in Immunoprecipitation applications (Heid, H., et. al. (2014). PLoS One. 9(2):e90386).

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Perilipin-1 in human breast and lipoma tissue sections.

Electron Microscopy Analysis: A representative lot detected Perilipin- positive lipid droplets by Immunoelectron Microscopy applications (Heid, H., et. al. (2014). PLoS One. 9(2):e90386).

Immunocytochemistry Analysis: A representative lot detected Perilipin-1 in Immunocytochemistry applications (Heid, H., et. al. (2014). PLoS One. 9(2):e90386).

Western Blotting Analysis: A representative lot detected Perilipin-1 in Western Blotting applications (Heid, H., et. al. (2014). PLoS One. 9(2):e90386).

Quality

Evaluated by Western Blotting in NIH3T3/L1 cell lysate.

Western Blotting Analysis: A 1:500 Dilution of this antibody detected Perilipin-1 in NIH3T3/L1 cell lysate.


Physical properties

~ 65 KDa observed; 55.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Human, Bovine

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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