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MABF146E

Sigma-Aldrich

Anti-CD45.1 (Mouse), violetFluor® 450, clone A20 Antibody

clone A20, from mouse, violetFluor® 450

Synonym(s):

Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen (L-CA), Lymphocyte antigen 5 (Ly-5), T200, CD45, CD45.1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

violetFluor® 450

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

A20, monoclonal

species reactivity

mouse

technique(s)

flow cytometry: suitable

UniProt accession no.

P06800

target post-translational modification

unmodified

Gene Information

mouse ... Ptprc(19264)

General description

The A20 antibody reacts with mouse CD45.1, also known as Ly5.1, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors.The A20 antibody is typically used as a leukocyte marker in Ly5.1 mouse strains: SJL/J, DA, STS/A and RIII. The antibody has been demonstrated to specific for CD45.1 and is not cross-reactive with CD45.2 bearing cells.

Application

Anti-CD45.1 (Mouse), violetFluor® 450, clone A20 Antibody is an antibody against CD45.1 for use in Flow Cytometry.

Quality

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.5 μg from a representative lot detected CD45.1 in one million SJL splenocytes.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

violetFluor is a registered trademark of Tonbo Biotechnologies

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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