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Millipore

Human Leptin ELISA Kit

measures and quantify Leptin levels in 25 μL serum or plasma

Synonym(s):

Leptin, Obesity factor

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.32

product name

Human Leptin "Dual Range" ELISA, This Human Leptin "Dual Range" ELISA is used to measure & quantify Leptin levels in Metabolism & Endocrine & Neuroscience research.

Quality Level

200

species reactivity

human

packaging

kit of 1 × 96 wells

parameter

25 μL sample volume (4hr assay)

assay range

accuracy: 94-114%
intra-assay cv: 1.4-4.9
(Sensitive Assay)
intra-assay cv: 2.6-4.6
linearity: 80-105%
linearity: 96-125%
(sensitive assay)
sensitivity: 0.125 ng/mL
(sensitive assay)
sensitivity: 0.125-20 ng/mL
sensitivity: 0.5 ng/mL
standard curve range: 0.5-100 ng/mL

technique(s)

ELISA: suitable

input

sample type cell culture supernatant
sample type plasma (K2 EDTA)
sample type serum

NCBI accession no.

NM_000230.1

UniProt accession no.

P41159

application(s)

research use

detection method

colorimetric (450nm/590nm)

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... LEP(3952)

Related Categories

General description

Leptin is a hormone that is secreted by the adipose tissue. It is also produced by the gastrointestinal tract and placenta. Leptin consists of a four-helix bundle. Leptin participates in regulating appetite, body weight, and energy expenditure. Excessive leptin is associated with cardiovascular complications like hypertension, coronary heart disease, and stroke. The LEP gene is mapped on the human chromosome at 7q32.1. Human Leptin "Dual Range" enzyme-linked immunosorbent assay (ELISA) kit is used for the non-radioactive quantification of human leptin in serum, plasma, and other biological media.

Application

Human Leptin "Dual Range" ELISA kit has been used in the analysis of leptin in:
  • blood samples
  • serum samples
  • plasma samples

Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Warning

Hazard Statements

H290,H317

Hazard Classifications

Met. Corr. 1 - Skin Sens. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Seungah Lee et al.
Talanta, 78(2), 608-612 (2009-02-11)
We report a method for the quantitative analysis of human serum leptin, which is a protein hormone associated with obesity, using a nanoarray protein chip based on a single-molecule sandwich immunoassay. The nanoarray patterning of a biotin-probe with a spot
A high-legume low-glycemic index diet reduces fasting plasma leptin in middle-aged insulin-resistant and -sensitive men.
Zhang Z, Lanza E, Ross AC, Albert PS, Colburn NH, Rovine MJ, Bagshaw D, Ulbrecht JS, Hartman TJ
European Journal of Clinical Nutrition null
Preoperative serum leptin levels in patients with breast cancer.
Mehmet Aliustaoglu,Ahmet Bilici,Mahmut Gumus,Aslihan Topal Colak,Gulcan Baloglu et al.
Medical Oncology (Northwood, London, England) null
P Prolo et al.
The international journal of biochemistry & cell biology, 30(12), 1285-1290 (1999-01-30)
Leptin is an adipocyte hormone that signals nutritional status to the central nervous system (CNS) and peripheral organs. Leptin is also synthetized in the placenta and in gastrointestinal tract, although its role in these tissues is not yet clear. Circulating
Metabolic state alters economic decision making under risk in humans.
Symmonds, Mkael, et al.
Testing, 5, e11090-e11090 (2010)
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