480352

2.5S NGF, Mouse Submaxillary Glands

• Synonym(s): 2.5S-Nerve Growth Factor
• UNSPSC Code: 12352202

Properties

• Assay: ≥95% (HPLC)
• Quality Level: 100
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Native 2.5S nerve growth factor from mouse submaxillary glands. Enhances survival, phagocytosis, and generation of superoxide in murine neutrophils. Reported to stimulate extracellular matrix invasion by human myeloma cells.

Native 2.5S nerve growth factor from mouse submaxillary glands. Enhances the survival, phagocytosis, and generation of superoxide in murine neutrophils. Reported to stimulate extracellular matrix invasion by human myeloma cells.

Biochem/physiol Actions

ED₅₀ ≤ 15 ng/ml using a PC-12 neurite outgrowth assay

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from sterile-filtered PBS.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 weeks at -20°C.

Reconstitute to ≥10 µg/ml in sterile H₂O or buffer containing 2% BSA.

Other Notes

Hermann, J.L., et al. 1993. Mol. Cell. Biol. 4, 1205.
Raffioni, S., and Bradshaw, R.A. 1992. Proc. Natl. Acad. Sci. USA89, 9121.
Traverse, S., et al. 1992. Biochem. J.288, 351.
Levi-Montalcini, R. 1987. In Vitro Cell. Dev. Biol.23, 227.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1