Developed specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels.
Developed specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels. Typical concentrations in these solutions are 0.5 - 10 mg/L. EtBr is bound and concentrated by the adsorber column, allowing removal of the filtrate. Each column normally binds at least 300 mg of ethidium bromide from TAE or TBE buffers. Residual capacity is easily visualized because the EtBr appears as a dark red to black band on the column. As long as this band has not reached the bottom of the column bed, the concentration of dye is still below the column capacity.
Developed specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels. Typical concentrations in these solutions are 0.5-10 mg/l. EtBr is bound and concentrated by the adsorber column, allowing removal of the filtrate. Each column normally binds at least 300 mg of ethidium bromide from TAE or TBE buffers. Residual capacity is easily visualized because the EtBr appears as a dark red to black band on the column. As long as this band has not reached the bottom of the column bed, the concentration of dye is still below the column capacity.
Biochem/physiol Actions
Cell permeable: no
Primary Target Removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers
Product does not compete with ATP.
Reversible: no
Warning
Toxicity: Irritant (B)
Reconstitution
When the adsorber column is not in use, both ends should be closed off. The column should not be allowed to dry out, as air bubbles may form in the column, hindering flow rate.
Other Notes
MacGregor, J.T., and Johnson, I.J. 1977. Mutat. Res.48, 103. Waring, M. 1975. In: Antibiotics, Corcoran, J.W., and Hahn, F.E., Editors, Vol. 3, 141, Cold Spring Harbor, NY.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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