204896

Complement C8, Human

Complement C8, Human, CAS 80295-58-5, is a native glycoprotein composed of three non-identical subunits of 64, (α), 64 (β), and 22 kDa (γ).

• Synonym(s): Complement C8, Human
• CAS Number: 80295-58-5
• MDL number: MFCD00130847
• UNSPSC Code: 12352202
• NACRES: NA.25

Properties

• Quality Level: 200
• Assay: ≥85% (SDS-PAGE)
• form: liquid
• specific activity: ≥150,000 C8H50 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• impurities: IgG, IgA, IgM, albumin, C3, C4, C7, C9, trace
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

Native, human C8 complement component. Glycoprotein composed of three non-identical subunits of M.W. 64 kDa (α), 64 kDa (β), and 22 kDa (γ). Present in normal human serum at 55 µg/ml. On activation of complement via either the classical or alternative pathway, C5 is cleaved into C5a and C5b fragments. The nascent C5b fragment binds to C6, C7, and C8 resulting in formation of the C5b-8 complex on target surfaces. Each membrane-bound, hydrophobic C5b-8 complex combines with three to six C9 molecules to complete the assembly of the lytic C5b-9 complement membrane attack complex (MAC).

Native, human C8 complement component. Glycoprotein composed of three non-identical subunits of M.W. 64,000 (α), 64,000 (β), and 22,000 (γ). Present in normal human serum at 55 µg/ml. On activation of complement via either the classical or alternative pathway, C5 is cleaved into C5a and C5b fragments. The nascent C5b fragment binds to C6, C7, and C8, resulting in formation of C5b-8 complex on target surfaces. Each membrane-bound, hydrophobic C5b-8 complex combines with three to six C9 molecules to complete the assembly of the lytic C5b-9 complement membrane attack complex (MAC).

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS, pH 7.2.

Preparation Note

Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Steckel, E.W., et al. 1980. J. Biol. Chem. 255, 11997.
Petersen, B.H., et al. 1976. J. Clin. Invest. 57, 283.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• WGK: WGK 2
• Flash Point(F): does not flash
• Flash Point(C): does not flash