14-770-M

mTOR (1362-end) Protein, active, 10 µg

Active, N-terminal FLAG-tagged, recombinant, human mTOR, amino acids 1362-end, for use in Kinase Assays.

• Synonym(s): FRAP1
• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.41

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in baculovirus infected Sf21 cells
• form: liquid
• specific activity: 377 U/mg
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable (kinase)
• solubility: water: soluble
• NCBI accession no.: NM_004958.2
• UniProt accession no.: P42345
• storage temp.: −70°C
• Gene Information: human ... MTOR(2475)

Description

General description

Research area: Cell Signaling

Protein Target: mTOR (FRAP1)

Target Sub-Family: Lipid/Atypical

N-terminal FLAG-tagged, recombinant, human mTOR, amino acids 1362-end

The mechanistic target of rapamycin (mTOR) protein is a serine/threonine protein kinase belonging to PI3K-related kinase (PIKK) family, expressed throughout the body.[1][2]

Application

The active mTOR (1362-end) protein, 10 µg, has been used in the mTOR substrate phosphorylation assay to validate the biochemical and pharmacological profile of a novel mTOR-KI for targeting mTOR signaling pathways in vitro and in vivo.[3]

Biochem/physiol Actions

Mechanistic target of rapamycin (mTOR) plays a pivotal role in regulating various essential cellular processes, ranging from protein synthesis to autophagy.[2] Moreover, it influences metabolism, cellular survival, gene transcription, and cytoskeletal components. It gets activated in response to growth factors, nutrients, mitogens, and hormones.[1]Downregulation of mTOR signaling is associated with the advancement of cancer and diabetes, and aging process.[2]

Packaging

Also available in 100μg size, please inquire for pricing and availability.

Unit Definition

Specific Activity: where one unit of mTOR activity is defined as 1 nmole of phosphate incorporated into 2mg/ml mTOR substrate per minute at 30°C with a final ATP concentration of 100µM.

Physical form

16µg of enzyme in 100µl of 10mM HEPES, 50mM NaCl, 50mM β-glycerophosphate, 0.25mM sodium orthovanadate, 10mM NaF, 300µg/ml FLAGTM peptide, 20% v/v glycerol

Storage and Stability

Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2