07-627
Anti-Histone H2A.X Antibody
serum, Upstate®
Synonym(s):
H2A histone family, member X, H2AX histone
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
inhibition assay: suitable (peptide)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... H2AC21(317772)
General description
Histone H2AX (UniProt: P16104; also known as H2a/x, Histone H2A.X) is encoded by the H2AFX (also known as H2AX) gene (Gene ID: 3014) in human. Histones are highly conserved basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. They play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes, which limits DNA accessibility to the cellular machineries that require DNA as a template. The histone H2A.X is a variant member of the H2A family of histones that is distinguished from other H2A histones by a unique carboxy-terminal sequence. This C-terminal tail becomes phosphorylated following double-stranded DNA breaks following irradiation or apoptosis. This unique sequence is highly conserved throughout eukaryotic evolution and is rapidly phosphorylated by ATM or ATR in response to DNA double-strand breaks. H2A.X phosphorylation is important in the formation of a stable repair complex at the site of DNA damage.
Specificity
This rabbit polyclonal antibody detects Histone H2AX in human cells. It targets an epitope with in 15 amino acids from the C-terminal region.
Immunogen
KLH-conjugated, synthetic peptide (CSATVGPKAPSGGKKA) corresponding to amino acids 121-135 of human histone H2A.X, with an N-terminal cysteine added for conjugation purposes. The immunizing sequence has 13/15 identical amino acids in mouse.
Application
Anti-Histone H2A.X Antibody, Cat. No. 07-627, is a highly specific rabbit polyclonal antibody that targets Histone H2A.X and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Histone H2A.X in human pancreas and human colon tissue sections.
Western Blotting Analysis: A 1:500 dilution from a representative lot detected Histone H2A.X in H2A and H2A.X recombinant protein.
Western Blotting Analysis: A 1:500 dilution from a representative lot detected Histone H2A.X in H2A and H2A.X recombinant protein.
Quality
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Histone H2A.X in Jurkat cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Histone H2A.X in Jurkat cell lysate.
Target description
~15 kDa; 15.15 kDa calculated.
Physical form
Rabbit antiserum containing 0.05% sodium azide and 30% glycerol. Liquid at -20°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Identification of functionally relevant histidine residues in the apoptotic nuclease CAD.
G Meiss,S R Scholz,C Korn,O Gimadutdinow,A Pingoud
Nucleic Acids Research null
Mitochondrial reactive oxygen species trigger hypoxia-inducible factor-dependent extension of the replicative life span during hypoxia.
Bell, EL; Klimova, TA; Eisenbart, J; Schumacker, PT; Chandel, NS
Molecular and cellular biology null
Telomerase inhibitor Imetelstat (GRN163L) limits the lifespan of human pancreatic cancer cells.
Burchett, KM; Yan, Y; Ouellette, MM
Testing null
H2AX chromatin structures and their response to DNA damage revealed by 4Pi microscopy.
Bewersdorf, J; Bennett, BT; Knight, KL
Proceedings of the National Academy of Sciences of the USA null
Zhijun Qiu et al.
Scientific reports, 7, 43156-43156 (2017-02-23)
Gross chromosomal rearrangements (GCRs), including translocations, inversions amplifications, and deletions, can be causal events leading to malignant transformation. GCRs are thought to be triggered by DNA double strand breaks (DSBs), which in turn can be spontaneous or induced by external
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