N9003

4-Nitrophenyl N-acetyl-β-D-galactosaminide

≥98%

• Synonym(s): 4-Nitrophenyl 2-acetamido-2-deoxy-β-D-galactopyranoside, p-Nitrophenyl N-acetyl-β-D-galactosaminide
• Empirical Formula (Hill Notation): C₁₄H₁₈N₂O₈
• CAS Number: 14948-96-0
• Molecular Weight: 342.30
• Beilstein: 96194
• EC Number: 239-024-3
• MDL number: MFCD00067360
• UNSPSC Code: 12352204
• PubChem Substance ID: 24897884
• NACRES: NA.28

Properties

• Quality Level: 200
• Assay: ≥98%
• form: powder
• solubility: DMF: 50 mg/mL
• storage temp.: −20°C
• SMILES string: CC(=O)N[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1Oc2ccc(cc2)[N+]([O-])=O
• InChI: 1S/C14H18N2O8/c1-7(18)15-11-13(20)12(19)10(6-17)24-14(11)23-9-4-2-8(3-5-9)16(21)22/h2-5,10-14,17,19-20H,6H2,1H3,(H,15,18)/t10-,11-,12+,13-,14-/m1/s1
• InChI key: OMRLTNCLYHKQCK-RKQHYHRCSA-N

Description

General description

4-Nitrophenyl N-acetyl-β-D-galactosaminide is a chromogenic substrate for enzymes N-acetyl-β-D-galactosaminidase and N-Acetyl-β-D-hexosaminidase. It is membrane-permeable and serves as a substrate for the enzyme 6-sulfotransferase in Golgi vesicles.

Application

4-Nitrophenyl N-acetyl-β-D-galactosaminide has been used:

• as a synthetic substrate in the glycosyl hydrolase assay of Yersinia pseudotuberculosis protein
• as a substrate in -β-hexosaminidase assay in adenocarcinomic human alveolar basal epithelial cells A549
• as a substrate for N-acetyl-β-D-galactosaminidase assay in rat kidney tissue extracts

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves