P3397

Phosphoglucomutase from rabbit muscle

ammonium sulfate suspension, ≥100 units/mg protein

• Synonym(s): PGM, α-D-Glucose-1,6-bisphosphatase, α-D-Glucose-1-phosphate phosphotransferase
• CAS Number: 9001-81-4
• Enzyme Commission number: 5.4.2.2 (BRENDA, IUBMB)
• MDL number: MFCD00131886
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: rabbit muscle
• description: ammonium sulfate suspension, >=100 units/mg protein
• form: ammonium sulfate suspension
• specific activity: ≥100 units/mg protein
• storage condition: (Tightly closed)
• technique(s): activity assay: suitable
• color: white to light yellow
• foreign activity: lactic dehydrogenase ≤0.5%; phosphoglucose isomerase ≤0.01%; pyruvate kinase ≤0.05%
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Research area: Cell Signaling

Phosphoglucomutase (PGM), a conserved enzyme, is abundantly found in animals, plants, and microorganisms. It is distributed in almost all tissues.

Application

Phosphoglycomutase has been used:

• to study glycogenesis and phosphoglycomutase deficiency in humans
• in phosphoglucomutase assays to study metabolic regulation
• for assay A to assay enzymes closely linked to glycogen metabolism in transformed BL21(DE3)C43 cells
• in sucrose synthase (SuSy) andADPglucose pyrophosphorylase (AGPase) assays(3)
• in enzyme activity assay for coupling the reduction of nicotinamide adenine dinucleotide phosphate (NADP⁺)to determine glucose-1-phosphate from sucrose and inorganic phosphate (Pi)

Biochem/physiol Actions

Phosphoglucomutase(PGM) enzyme plays a vital role in glycolysis and gluconeogenesis. It is involved in glycogen and trehalose metabolism in insects. PGM participates in the development of plants and some microorganisms. It is essential for proteins, lipids, and nucleic acid metabolism and is crucial for the development of plants because glucose-6-phosphate is a crucial central metabolite. PGM catalyzes the interconversion of glucose-6-phosphate (G-6-P)and glucose-1-phosphate (G-1-P).

Unit Definition

One unit will convert 1.0 μmole of α-D-glucose 1-phosphate to α-D-glucose 6-phosphate per min at pH 7.4 at 30 °C.

Physical form

Crystalline suspension in 3.2 M (NH₄)₂SO₄, pH 6.0 containing 0.01% EDTA

Analysis Note

Protein determined by biuret.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves