07-729
Anti-CTCF Antibody
serum, Upstate®
Synonym(s):
11 zinc finger transcriptional repressor, 11-zinc finger protein, CCCTC-binding factor, CCCTC-binding factor (zinc finger protein), CTCFL paralog, transcriptional repressor CTCF
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human, canine, rat, primate
packaging
antibody small pack of 25 μL
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable (ChIP-seq)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... CTCF(10664)
General description
CTCF is a ubiquitous 11 zinc finger (ZF) protein with highly versatile functions. A transcriptional repressor, CTCF, binds to promoters of vertebrate c-myc gene. It also binds to the PLK and PIM1 promoters. CTCF may prevent the access of transcriptional activators to enhancers. CTCF also acts as a transcriptional activator of APP. It is involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. CTCF may also act as tumor suppressor. CTCF organizes epigenetically controlled chromatin insulators that regulate imprinted genes in soma.
Specificity
Predicted to cross-react with mouse, rat, dog and primates based on sequence homology.
Recognizes CTCF at a.a. 659-675.
Immunogen
KLH-conjugated, synthetic peptide corresponding to amino acids 659-675 (C-TNQPKQNQPTAIIQVED) of human CCCTC-binding factor (CTCF) with a N-terminal cysteine added for conjugation purposes.
Application
Chromatin Immunoprecipitation:
An independent laboratory has confirmed that this antibody immunoprecipitates CTCF-bound chromatin from WEHI B-cells.
An independent laboratory has confirmed that this antibody immunoprecipitates CTCF-bound chromatin from WEHI B-cells.
Use Anti-CTCF Antibody (Rabbit Polyclonal Antibody) validated in ChIP, WB, ChIP-seq to detect CTCF also known as 11-zinc finger protein, CCCTC-binding factor.
Quality
Routinely evaluated by Western Blot.
Western Blot Analysis:
A 1:1000–1:5000 dilution of this lot detected CTCF in HeLa nuclear extract. A previous lot detected CTCF in K562 nuclear extract (data not shown).
Western Blot Analysis:
A 1:1000–1:5000 dilution of this lot detected CTCF in HeLa nuclear extract. A previous lot detected CTCF in K562 nuclear extract (data not shown).
Target description
Approx. 120 kDa
Linkage
Replaces: MABE547
Physical form
Rabbit polyclonal antiserum containing 0.05% sodium azide and 30% glycerol.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analysis Note
Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 µL of extract and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced extract per lane for minigels.
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 µL of extract and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced extract per lane for minigels.
Other Notes
Concentration: The concentration of this serum antibody has not been determined.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Role of CCCTC binding factor (CTCF) and cohesin in the generation of single-cell diversity of protocadherin-? gene expression.
Monahan, K; Rudnick, ND; Kehayova, PD; Pauli, F; Newberry, KM; Myers, RM; Maniatis, T
Proceedings of the National Academy of Sciences of the USA null
Occupancy of chromatin organizers in the Epstein-Barr virus genome.
Holdorf, MM; Cooper, SB; Yamamoto, KR; Miranda, JJ
Virology null
M D Delgado et al.
FEBS letters, 444(1), 5-10 (1999-02-26)
CTCF is a transcriptional repressor of the c-myc gene. Although CTCF has been characterized in some detail, there is very little information about the regulation of CTCF activity. Therefore we investigated CTCF expression and phosphorylation during induced differentiation of human
Friedreich ataxia patient tissues exhibit increased 5-hydroxymethylcytosine modification and decreased CTCF binding at the FXN locus.
Al-Mahdawi, S; Sandi, C; Mouro Pinto, R; Pook, MA
Testing null
An elaborate pathway required for Ras-mediated epigenetic silencing.
Gazin, C; Wajapeyee, N; Gobeil, S; Virbasius, CM; Green, MR
Nature null
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