コンテンツへスキップ
Merck
  • Dopaminergic and glutamatergic microdomains in a subset of rodent mesoaccumbens axons.

Dopaminergic and glutamatergic microdomains in a subset of rodent mesoaccumbens axons.

Nature neuroscience (2015-02-11)
Shiliang Zhang, Jia Qi, Xueping Li, Hui-Ling Wang, Jonathan P Britt, Alexander F Hoffman, Antonello Bonci, Carl R Lupica, Marisela Morales
要旨

Mesoaccumbens fibers are thought to co-release dopamine and glutamate. However, the mechanism is unclear, and co-release by mesoaccumbens fibers has not been documented. Using electron microcopy, we found that some mesoaccumbens fibers have vesicular transporters for dopamine (VMAT2) in axon segments that are continuous with axon terminals that lack VMAT2, but contain vesicular glutamate transporters type 2 (VGluT2). In vivo overexpression of VMAT2 did not change the segregation of the two vesicular types, suggesting the existence of highly regulated mechanisms for maintaining this segregation. The mesoaccumbens axon terminals containing VGluT2 vesicles make asymmetric synapses, commonly associated with excitatory signaling. Using optogenetics, we found that dopamine and glutamate were released from the same mesoaccumbens fibers. These findings reveal a complex type of signaling by mesoaccumbens fibers in which dopamine and glutamate can be released from the same axons, but are not normally released at the same site or from the same synaptic vesicles.

材料
製品番号
ブランド
製品内容

Sigma-Aldrich
抗チロシンヒドロキシラーゼ抗体, Chemicon®, from rabbit
Sigma-Aldrich
抗チロシンヒドロキシラーゼ抗体、クローンLNC1, ascites fluid, clone LNC1, Chemicon®
Sigma-Aldrich
抗チロシンヒドロキシラーゼ抗体, Chemicon®, from sheep
Sigma-Aldrich
抗Myc Tag抗体、クローン4A6, clone 4A6, Upstate®, from mouse
Sigma-Aldrich
抗小胞グルタミン酸トランスポーター2抗体, clone 8G9.2, Chemicon®, from mouse
Sigma-Aldrich
抗小胞モノアミントランスポーター2抗体, Chemicon®, from rabbit
Sigma-Aldrich
抗VMAT2/SLC18A2 ヤギ宿主抗体, affinity isolated antibody, buffered aqueous solution