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Molybdenum-containing membrane-bound formate dehydrogenase isolated from Citrobacter sp. S-77 having high stability against oxygen, pH, and temperature.

Molybdenum-containing membrane-bound formate dehydrogenase isolated from Citrobacter sp. S-77 having high stability against oxygen, pH, and temperature.

Journal of bioscience and bioengineering (2014-04-23)

Nga T Nguyen, Takeshi Yatabe, Ki-Seok Yoon, Seiji Ogo

要旨

Membrane-bound formate dehydrogenase (FDH) was purified to homogeneity from a facultative anaerobic bacterium Citrobacter sp. S-77. The FDH from Citrobacter sp. S-77 (FDHS77) was a monomer with molecular mass of approximately 150 kDa. On SDS-PAGE, the purified FDHS77 showed as three different protein bands with molecular mass of approximately 95, 87, and 32 kDa, respectively. Based on the N-terminal amino acid sequence analysis, the sequence alignments observed for the 87 kDa protein band were identical to that of the large subunit of 95 kDa, indicating that the purified FDHS77 consisted of two subunits; a 95 kDa large subunit and a 32 kDa small subunit. The purified FDHS77 in this purification did not contain a heme b subunit, but the FDHS77 showed significant activity for formate oxidation, determined by the Vmax of 30.4 U/mg using benzyl viologen as an electron acceptor. The EPR and ICP-MS spectra indicate that the FDHS77 is a molybdenum-containing enzyme, displaying a remarkable O2-stability along with thermostability and pH resistance. This is the first report of the purification and characterization of a FDH from Citrobacter species.

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モリブデン, foil, 15mm disks, thickness 0.005mm, 99.9%

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モリブデン, powder, <150 μm, 99.9% trace metals basis

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モリブデン, foil, 25mm disks, thickness 0.0125mm, 99.9%

モリブデン, rod, 200mm, diameter 6.0mm, centerless ground, 99.9%

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モリブデン, foil, thickness 0.025 mm, ≥99.9% trace metals basis

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モリブデン, foil, thickness 0.1 mm, ≥99.9% trace metals basis

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モリブデン, powder, <150 μm, 99.99% trace metals basis

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モリブデン, powder, 10 μm, ≥99.95% trace metals basis

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モリブデン, nanopowder, <100 nm particle size (TEM), 99.8% trace metals basis

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モリブデン, wire, diam. 1.0 mm, 99.95% trace metals basis

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モリブデン, foil, thickness 1.0 mm, ≥99.9% trace metals basis

モリブデン, pellets, 100g, max. size 10mm, 99.9%

モリブデン, foil, 150x150mm, thickness 1.0mm, annealed, 99.98%

モリブデン, wire, straight, 1000mm, diameter 0.25mm, 99.95%

モリブデン, foil, light tested, 100x100mm, thickness 0.01mm, 99.9%

モリブデン, pellets, 200g, max. size 10mm, 99.9%

モリブデン, rod, 50mm, diameter 5.0mm, 99.98%

モリブデン, mesh, 100x100mm, nominal aperture 0.44mm, wire diameter 0.07mm, 50 wires/inch, open area 67%, plain weave mesh

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モリブデン, rod, 100mm, diameter 2.0mm, centerless ground, 99.9%

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モリブデン, insulated wire, 10m, conductor diameter 0.125mm, insulation thickness 0.012mm, polyimide insulation, 99.95%