コンテンツへスキップ
Merck
  • P2X7 receptor is essential for cross-dressing of bone marrow-derived dendritic cells.

P2X7 receptor is essential for cross-dressing of bone marrow-derived dendritic cells.

iScience (2021-12-25)
Carlos Barrera-Avalos, Pedro Briceño, Daniel Valdés, Mónica Imarai, Elías Leiva-Salcedo, Leonel E Rojo, Luis A Milla, Juan Pablo Huidobro-Toro, Claudia Robles-Planells, Alejandro Escobar, Francesco Di Virgilio, Gabriel Morón, Daniela Sauma, Claudio Acuña-Castillo
要旨

T cell activation requires the processing and presentation of antigenic peptides in the context of a major histocompatibility complex (MHC complex). Cross-dressing is a non-conventional antigen presentation mechanism, involving the transfer of preformed peptide/MHC complexes from whole cells, such as apoptotic cells (ACs) to the cell membrane of professional antigen-presenting cells (APCs), such as dendritic cells (DCs). This is an essential mechanism for the induction of immune response against viral antigens, tumors, and graft rejection, which until now has not been clarified. Here we show for first time that the P2X7 receptor (P2X7R) is crucial to induce cross-dressing between ACs and Bone-Marrow DCs (BMDCs). In controlled ex vivo assays, we found that the P2X7R in both ACs and BMDCs is required to induce membrane and fully functional peptide/MHC complex transfer to BMDCs. These findings show that acquisition of ACs-derived preformed antigen/MHC-I complexes by BMDCs requires P2X7R expression.

材料
製品番号
ブランド
製品内容

Millipore
G 418 Sulfate, Cell Culture Tested, G418 also known as Geneticin is an aminoglycoside antibiotic related to Gentamicin. Used as a selective agent in transfection of eukaryotic cells. Has highest potency ≥730 µg/mg and purity ≥98%.
ログイン組織・契約価格を表示する
カタログ番号容量在庫状況単価数量
200 μg
出荷予定日 2025年3月23日
お客様のターゲット用に防腐剤フリーのリコンビナント抗体を利用できます。ZRB1097をお試しください
$609.00